傣百解对肠道黏膜屏障功能的保护作用及机制研究

Study on the Protective Effect and Mechanism of Daibaijie on Intestinal Mucosal Barrier Function

目的观察傣百解对肠道黏膜屏障功能的保护作用及作用机制。方法IEC-6细胞分为空白对照组、LPS组、傣百解不同剂量组、LPS+傣百解不同剂量组,分别检测傣百解干预后对IEC-6细胞及LPS诱导后IEC-6细胞模型的活力和炎症因子的水平。另取昆明种小鼠随机分为正常对照组、LPS组、LPS+傣百解高、低剂量组,连续给药1周后,建立小鼠肠道屏障损伤模型,分别检测血清中D-乳酸含量和结肠中TNF-α、IL-1β、IL-6的mRNA表达水平,并观察结肠病理变化。结果傣百解在30~480μg/mL范围内对正常IEC-6细胞活力无明显影响,且能对抗LPS对IEC-6细胞活力的抑制作用,降低LPS诱导后IEC-6细胞中的IL-1β、TNF-α、IL-6水平。小鼠肠道损伤模型结果显示傣百解能够降低模型小鼠血清中D-乳酸含量和结肠中IL-6、IL-1β、TNF-αmRNA表达水平,同时减少结肠黏膜的损伤。结论傣百解对肠道黏膜屏障功能具有保护作用,其作用机制可能与提高IEC-6细胞活力及减轻炎症反应有关。

Objective To observe the protective effect and mechanism of Daibaijie on intestinal mucosal barrier function.Methods IEC-6 cells were randomly divided into blank control group,LPS group,different dose groups of Daibaijie,LPS+different dose groups of Daibaijie.The cell viability and inflammatory factor levels of IEC-6 cells and IEC-6 cell model induced by LPS were detected after Daibaijie intervention.In addition,Kunming mice were randomly divided into normal control group,LPS group,LPS+Daibaijie high and low dose groups.After one week of continuous administration,the intestinal barrier injury model of mice was established.The content of D-lactate in serum and the mRNA expression levels of TNF-α,IL-1βand IL-6 in colon were detected,pathology of colon were also observed.Results In the range of 30-480μg/mL,Daibaijie had no significant effect on the cell viability of IEC-6 cells,and could resist the inhibition of LPS on the cell viability of IEC-6 cells,and reduce the levels of IL-1β,TNF-αand IL-6 in IEC-6 cells after LPS induction.Daibaijie could also reduce the levels of D-lactate in LPS induced animal model serum and the mRNA expressions of IL-6,IL-1βand TNF-αin colon,reduce the injury of colon.Conclusion Daibaijie has protective effect on intestinal mucosal barrier function,and the mechanism may be related to improving IEC-6 cell viability and reducing inflammatory response.