跑台运动联合螺旋藻多糖对阿尔茨海默病模型小鼠认知功能及同型半胱氨酸水平的影响

The effects of exercise combined with spirulina polysaccharide on cognitive function and homocysteine level in mice with Alzheimer disease

目的:探讨跑台运动联合螺旋藻多糖对阿尔茨海默病(AD)模型小鼠认知功能及同型半胱氨酸水平的影响。方法:实验小鼠腹腔注射亚硝酸钠100mg/kg和D-半乳糖120mg/kg制备阿尔茨海默病模型,将建模成功的32只小鼠随机分为模型组(A),运动组(B1),药物组(B2),联合组(B3),每组8只,对运动组、药物组、联合组分别进行运动干预、药物干预及联保干预,B1组每天以10m/min跑200m,B2组给予螺旋藻多糖灌胃(67mg/kg/d),B3组运动联合螺旋藻多糖灌胃(B1+B2),连续8周;正常组(C)小鼠8只作为对照组,给予等量生理盐水注射,连续注射8周,第9周时,采用Morris水迷宫检测所有小鼠的学习记忆能力后取脑部组织匀浆,测同型半胱氨酸(Hcy)、S100β蛋白水平。结果:与A组相比:C、B2、B3组120S内跨越平台的次数和在目标象限的探索时间均差异显著(P<0.05);C、B1、B2、B3组Hcy含量减少,差异显著(P<0.05);C组、B3组S100β差异显著(P<0.05)。结论:跑台运动联合螺旋藻多糖可有效改善AD模型小鼠的认知功能,显著降低其脑部同型半胱氨酸水平。

Objective:to investigate the effect of exercise combined with spirulina polysaccharides on homocysteine levels in Alzheimer’s disease mice.Methods:The model mice of Alzheimer’s disease were prepared by intraperitoneal injection of sodium nitrite 100 mg/kg and d-galactose 120 mg/kg.Animal mice were randomly divided into the model group(group A),the experimental group(B1,B2)and normal group(C),each group of8,the experimental group exercise intervention,drug intervention respectively,B1 every day with 10 m/min to run 200 meters,B2 was given spiral alga of lavage(67 mg/kg/d),and B3 movement joint helix alga of lavage(B1 and B2).In the 9 th week,the Morris water maze was used to test the learning and memory ability of mice,and then brain tissue homogenate was taken to measure the levels of homocysteine(Hcy)and S100 protein.Results:Compared with A:the number of platform crossings and exploration time in the target quadrant of C,B2,and B3 were significantly different within 120 S(P<0.05).The content of Hcy in C,B1,B2 and B3 decreased,with a statistically significant difference(P<0.05).There were statistically significant differences in S100βbetween C and B3(P<0.05).Conclusions:Treadmill exercise combined with spirulina polysaccharide can effectively improve the cognitive function of AD model mice and significantly reduce the homocysteine level in the brain.