荷叶碱通过上调SDF-1/CXCR4信号通路促进人主动脉内皮细胞增殖

Nuciferine promotes proliferation of human aortic endothelial cell by up-regulating SDF-1/CXCR4 signaling pathway

目的探讨荷叶碱是否通过调控基质细胞衍生因子1(SDF-1)/趋化因子受体4(CXCR4)信号通路影响动脉内皮细胞增殖。方法分别使用不同质量浓度(0 mg/L、10 mg/L、20 mg/L、50 mg/L、100 mg/L)的荷叶碱培养人主动脉内皮细胞(HAEC)24 h,通过RT-QPCR和Western blot技术分别检测各组细胞中SDF-1和CXCR4 mRNA及其蛋白的表达情况。采用体外内皮细胞小管形成实验观察不同质量浓度荷叶碱对HAEC增殖能力的影响。结果SDF-1、CXCR4蛋白和mRNA在荷叶碱组HAEC中表达升高,在50 mg/L荷叶碱组中表达最显著。荷叶碱组HAEC体外小管形成能力与荷叶碱呈浓度依赖性增强,在50 mg/L荷叶碱组中最明显。结论荷叶碱可能通过上调SDF-1/CXCR4信号通路的表达促进血管内皮细胞增殖。

Aim To investigate whether Nuciferine affect the proliferation of arterial endothelial cell by regulating SDF-1/CXCR4 signaling pathway.Methods Human aortic endothelial cell(HAEC)were co-cultured with Nuciferine of different concentrations(0 mg/L,10 mg/L,20 mg/L,50 mg/L,100 mg/L)for 24 hours.The expressions of SDF-1,CXCR4 mRNA and their proteins in each group were detected by RT-QPCR and Western blot techniques,respectively.The effects of different concentrations of Nuciferine on the colonization ability of HAEC were observed by endothelial cell tubule formation experiment in vitro.Results The expression of SDF-1/CXCR4 protein and mRNA increased in Nuciferine group,especially in 50 mg/L group.The tubule formation energy of HAEC in vitro was enhanced in a concentration-dependent manner in the Nuciferine group,which was the most obvious in the 50 mg/L group.Conclusion Nuciferine can promote the proliferation of vascular endothelial cell by up-regulating the expression of SDF-1/CXCR4 signaling pathway.