鸟氨酸脱羧酶抑制剂对天仙子生长和托品烷生物碱生物合成的影响

Effects of ornithine decarboxylase inhibitor on the growth of Hyoscyamus niger and the biosynthesis of tropine alkaloids

天仙子(Hyoscyamus niger)是重要的托品烷生物碱(tropane alkaloids,TAs)药源植物,鸟氨酸脱羧酶(ornithine decarboxylase,ODC)作为腐胺生物合成的关键酶在植物的生长发育及次生代谢中发挥着重要作用。但是ODC是否参与天仙子TAs的生物合成,目前尚不清楚。本研究利用2.5 mmol·L-1的ODC特异性抑制剂二氟甲基鸟氨酸(difluoromethylornithine,DFMO)处理3周龄天仙子植株,处理2周后对实验组和对照组植株的多胺、TAs和生物量分别进行了测定。测定结果表明,DFMO能够显著抑制天仙子生长和多胺及TAs的生物合成。本研究表明ODC在天仙子生长发育及TAs生物合成中发挥着重要的作用。

Hyoscyamus niger is an important medicinal plant producing tropane alkaloids(TAs).Ornithine decarboxylase(ODC),as a key enzyme for putrescine biosynthesis,plays an important role in plant growth and secondary metabolism.However,it is unclear whether ODC is involved in the biosynthesis of TAs in H.niger.In this study,2.5 mmol·L-1 ODC-specific inhibitor difluoromethylornithine(DFMO)was used to treat 3-weekold H.niger plants,and then biomass and metabolites were measured after two-week-treatment.The results showed that DFMO can significantly inhibit the growth and the biosynthesis of TAs and polyamines in H.niger.The above studies indicate that ODC plays an important role in the growth and TAs biosynthesis in H.niger.