超声提取樱叶多糖及其体外抗氧化研究

Study on the Ultrasound-assisted Extraction Craft of Polysaccharides from the Leaves of Cerasua serrulata and Its Antioxidant Activities in vitro

以响应面法优化樱叶中多糖的超声提取工艺,考察多糖的体外抗氧化活性。在单因素实验的基础上,采用Box-Behnken中心组合实验和响应面分析法,研究提取时间、超声波功率、料液比对樱叶多糖提取率的影响,建立影响因素与响应值之间的数学模型,确立最佳提取工艺。同时,以DPPH的自由基清除能力、还原Fe 3+能力和清除·OH自由基能力,验证樱叶多糖的抗氧化活性。结果显示,樱叶中多糖的最佳提取工艺为:提取时间30 min,液料质量比29∶1,超声功率75 W。抗氧化实验结果表明,当樱叶多糖达到最大质量浓度1.20 mg/mL时,樱叶多糖(超声)、樱叶多糖(煮沸)和维生素C对DPPH的清除率依次为77.6%,68.8%,62.4%,樱叶多糖(超声)、樱叶多糖(煮沸)对·OH自由基的清除率依次为88.1%,69.2%,对还原Fe 3+能力较强。该结果显示樱叶多糖有一定抗氧化活性,且樱叶多糖(超声)较樱叶多糖(煮沸)活性强。超声波提取樱叶多糖具有提取效率高、时间短的优点,从多糖抗氧化活性检测来看,樱叶超声提取优于煎煮法。

The study aimed to get optimized craft of polysaccharide extraction and antioxidant activities from Cerasua serrulata.Owing to single-factor experiment,Box-Behnken design and its response surface analysis,the main factors(extraction time,power of ultrasound,the ratio of solvent and material)affecting the extraction yield of polysaccharide were studied and mathematical model between the response value and factors was established.At the same time,the antioxidant activity of the polysaccharide was determined by DPPH’s ability to scavenge free radicals,reduce Fe 3+and scavenge·OH radicals.The optimal craft was as follows:ratio of solvent and material with 29∶1,extracting 30 min by 75 W.When the concentration of polysaccharides reached 1.20 mg/mL,the DPPH clearance rate of polysaccharides(extracted by ultrasound),polysaccharides(extracted by boiling method),vitamin C was 77.6%,68.8%,62.4%.The clearance rate of free radical·OH of polysaccharides(extracted by ultrasound)and polysaccharides(extracted by boiling method)was respectively 88.1%,69.2%.Polysaccharides extracted by ultrasound showed better Fe 3+reduction activity.Compared with the craft of traditional boiling extraction,ultrasound extraction technology showed higher extraction rate,took less time and attained more bioactive polysaccharides.