BMP-2与TBC复合的参数和骨诱导能力的动物活体内评价

In vivo evaluation of parameters and osteoinductive capacity for the BMP-2 and true bone ceramic composites

目的探讨骨形成蛋白-2(BMP-2)、异种煅烧骨(TBC)复合的剂量以及TBC的粒径对复合材料骨诱导能力的影响。方法制备TBC支架材料(粒径0.300~0.710 mm),分别与不同剂量的BMP-2复合(125μg/g、250μg/g、500μg/g),观察新骨生成情况;以0.710~1.400 mm、0.300~0.710 mm、0.150~0.300 mm 3种粒径TBC为支架材料,分别与500μg/g的BMP-2复合,观察新骨生成情况。结果BMP-2负载量为125μg/g时,无明显新骨生成;BMP-2负载量为250μg/g时,50%的标本观察到新骨生成;当BMP-2负载量为500μg/g时,所有标本均观察好的骨诱导能力。粒径越小,骨小梁数目明显减少,粒径在0.300~1.400 mm的复合材料新骨生成情况更好。结论通过控制BMP-2与TBC复合材料中BMP-2的剂量和TBC的粒径,可保证复合材料具有较好的骨诱导能力。

Objective To explore the dose of the bone morphogenetic protein-2(BMP-2)composited to the ture bone ceramic(TBC)and to investigate the particle size of TBC on the osteoinductive ability of TBC composites.Methods Fresh bovine cancellous bone was calcined to prepare TBC scaffold materials(particle size 0.300~0.710 mm).TBC scaffold materials were compounded with high,medium and low(125μg/g,250μg/g,500μg/g)different doses of BMP-2.TBC composites were implanted into the muscle space of mice to evaluate their osteoinductive activity.Three kinds of particle size TBC(0.710-1.400 mm,0.300-0.710 mm,0.150-0.300 mm)were compounded with 500μg/g BMP-2,respectively.And the new bone formation of the composite material was observed.Results Histological observation of HE showed that no new bone formation was observed when the BMP-2 load was 125μg/g.When the BMP-2 was loaded 250μg/g,new bone formation was observed in 50%of the samples.And when the BMP-2 load was 500μg/g,the new bone formation rate reached 100%.With the decrease of the particle size,the number of trabecular bone decreases significantly.The particle size of 0.300 mm-1.400 mm composite was conducive to the formation of new bone.Conclusion By controlling the dose of BMP-2 and the particle size of TBC in the TBC composites,it is possible to ensure that the composite has good osteoinductive ability.