高盐饮食对Dahl盐敏感大鼠肾脏基因表谱的影响

Effect of high-salt diet on gene expression profile of kidney in Dahl salt-sensitive rats

目的:通过基因芯片技术检测高盐饮食对盐敏感高血压模型Dahl盐敏感大鼠肾脏基因表达谱的影响。方法:将12只Dahl盐敏感大鼠和12只SS-13BN大鼠分别随机分为正常盐组、高盐组干预3周,在高盐组中各随机选取3只大鼠,分别应用Illumina大鼠表达谱芯片筛选检测肾脏差异表达基因,并对差异基因进行层次聚类、GO及Pathway功能分析,使用Real-time PCR对其中5个基因进行验证。结果:Dahl盐敏感大鼠高盐组与SS-13BN大鼠高盐组相比,有908个差异基因,其中上调基因429个,下调基因479个,对Mcoln3、SGK1、Slc34a2、Atp1a4、Trpv6等5个基因进行Real timePCR验证,结果与芯片结果抑制。经过GO功能分析发现,这些基因主要参与离子转运、能量代谢、脂肪酸代谢、氧化应激炎症、凋亡等过程。结论:Dahl盐敏感大鼠在盐负荷后肾脏不仅存在钠钾代谢相关通道基因表达异常,同时能量代谢、脂肪酸代谢、氧化应激炎症、凋亡等过程也存在调控失衡。

Objective: To investigate alteration of gene in kidney of Dahl rat using gene expression microarray analysis. Methods: Male Dahl sensitive rats and 13 BN-SS rats were randomly divided into nornal salt group and high salt group respectively;Illumina RatRef-12 expression beadchip were used for screening the renal different genes between high salt group and high salt plus potassium supplement group, and the clusters, GO, pathway analysis of functionally related genes were determined on the basis of their annotation term co-occurrence, and 5 different genes were verified by Real time PCR. Results: There were 429 upregulated genes and 479 downregulated genes between Dahl salt sensitive rats and 13 BN-SS rat in high salt group, totally 908 different genes;GO functional analysis found that these genes mainly take part in the process of ion transport, energy metabolism, fatty acid metabolism, oxidative stress, apoptosis and so on. 5 different genes including Mcoln3, SGK1, Slc34 a2, Atp1 a, Trpv6 were assessed, which were in in keeping with the result of microarray. Conclusions: Dahl salt-sensitive rats not only have abnormal gene expression of sodium and potassium metabolism-related channels in the kidneys after salt load, but also have imbalanced regulation of energy metabolism, fatty acid metabolism, oxidative stress inflammation, and apoptosis.