摘要
评估乙酰辅酶A羧化酶抗性突变对■唑酰草胺的抗性水平。[方法]采用CAPs或和dCAPs从不同菵草抗性种群中纯化出基因背景一致的纯合子和野生型,整株法测定其对■唑酰草胺的敏感性。[结果]Ile1781Leu、Trp1999Leu、Trp2027Cys、Ile2041Asn、Asp2078Gly、Gly2096Ala等突变对■唑酰草胺的抗性指数分别为5.4、12.2、90.8、1.0、8.9、1.1。[结论]菵草ACCase Ile1781Leu、Trp1999Leu、Trp2027Cys、Asp2078Gly等突变对■唑酰草胺有中等至高水平抗性,Ile2041Asn、Gly2096Ala等突变对■唑酰草胺没有抗性。
[Aims]This study aims to evaluate the resistant level to metamifop caused by known resistant mutations in acetyl co-enzyme carboxylase(ACCase).[Methods]The CAPs or dCAPs method was employed to get resistant homozygotes and susceptible wild type with same genetic ground back from resistant Beckmannia syzigachne populations.Then the whole-plant method was introduced to determine the resistant level to metamifop caused by these resistant mutations.[Results]The results showed that resistant index resulted from Ile1781Leu,Trp1999Leu,Trp2027Cys,Ile2041Asn,Asp2078Gly and Gly2096Ala mutations were 5.4,12.2,90.8,1.0,8.9 and 1.1,respectively.[Conclusions]The mutations of Ile1781Leu,Trp1999Leu,Trp2027Cys and Asp2078Gly led to medium to high level resistance to metamifop.The mutations of Ile2041Asn and Gly2096Ala did not show resistance to metamifop.
作者
刘炳奇
薛炜桦
白霜
李凌绪
罗小勇
LIU Bing-qi;XUE Wei-hua;BAI Shuang;LI Ling-xu;LUO Xiao-yong(College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,Shandong,China)
出处
《农药》
CAS
CSCD
北大核心
2020年第11期851-854,858,共5页
Agrochemicals
基金
山东省自然科学基金(ZR2019MC043)。