海藻渣中岩藻聚糖硫酸酯提取工艺优化

Optimization of extraction process of fucoidan sulfate from seaweed residue

以海藻渣为原料,采用酸提法提取岩藻聚糖硫酸酯,通过单因素试验和正交试验优化提取工艺条件,并结合Dische比色法筛选最优的乙醇沉淀体积分数,以实现岩藻聚糖硫酸酯粗品的纯化.结果表明:采用酸提法提取海藻渣中岩藻聚糖硫酸酯的最佳工艺条件为HCl浓度0.25mol/L,提取温度50℃,提取时间2h,料液比1:10,在此条件下,岩藻聚糖硫酸酯粗品的提取率为1.89%;结合Dische比色法筛选出最优的乙醇沉淀体积分数为70%,此时岩藻聚糖硫酸酯的质量浓度达到最大值,为3.12μg/mL.

Using seaweed residue as raw material,the fucoidan sulfate was extracted by acid extraction.The extraction process conditions were optimized through single factor experiment and orthogonal experiment.In order to achieve the purification of the crude fucoidan sulfate,the optimal ethanol precipitation volume fraction was screened in combination with the Dische colorimetric method.The results showed that the optimal process conditions for extracting fucoidan sulfate from seaweed residue by acid extraction were HCl concentration 0.25 mol/L,extraction temperature 50℃,extraction time 2 h,and material-liquid ratio 1:10.The extraction rate of crude fucoidan sulfate was 1.89%.Combined with the Dische colorimetric method,the optimal ethanol precipitation volume fraction was 70%.At this time,the mass concentration of fucoidan sulfate reached the maximum,which was 3.12μg/mL.