多发性骨髓瘤和淋巴瘤患者IgG亚类分析及其与血细胞参数的相关性

Analysis of IgG Subclass in Blood Serum between Multiple Myeloma and Lymphoma Patients and Its Correlation with Blood Cell Parameters

目的:探讨多发性骨髓瘤(multiple myloma,MM)和淋巴瘤(Lymphoma)患者免疫球蛋白G(immunoglobulin G,IgG)及其亚类的表达水平差异及与血细胞参数的相关性。方法:选择2019年1月-12月在四川省人民院确诊的MM患者129例和淋巴瘤患者113例,分别对总IgG和亚类IgG1、IgG2、IgG3和IgG4及与外周血常规部分参数进行回顾性分析。组间比较采用独立样本t检验或非参数Mann-Whitney U检验,IgG亚类与血细胞参数的关系采用相关分析,对于双变量正态分布资料则计算Pearson相关系数。对于双变量非正态分布资料则计算Spearman相关系数。结果:多发性骨髓瘤患者IgG1和IgG2水平明显高于淋巴瘤患者(P=0.001,P=0.000),IgG3和IgG4水平明显低于淋巴瘤患者(P=0.000,P=0.000),血清总IgG在2组间没有显著差异(P=0.717);按性别进行分组后的结果显示,男性多发性骨髓瘤和淋巴瘤患者IgG4含量均明显高于女性患者,差异具有统计学意义(Z=-3.191,P=0.001);多发性骨髓瘤患者的发病年龄、M%、E%明显高于淋巴瘤患者(P=0.000,P=0.005,P=0.019),而WBC、RBC、Hb明显低于淋巴瘤患者(P=0.013,P=0.000,P=0.014)。Spearman相关分析显示,随着多发性骨髓瘤患者血清中总IgG与IgG1含量的增高,RBC、Hb有降低趋势(r=-0.254,r=-0.272,r=-0.248和r=-0.289)。随着淋巴瘤患者血清中IgG4含量的增高,患者RBC、Hb有降低的趋势(r=-0.240,r=-0.251)。结论:IgG亚类检测及与血细胞参数的相关性研究对多发性骨髓瘤和淋巴瘤的诊断及发病机理的探讨等具有重要的临床意义。

Objective:To investigate the expression of immunoglobulin G(IgG)and its subclasses in patients with multiple myeloma(MM)and lymphoma(LYM)and its correlation with blood cell parameters.Methods:129 patients with multiple myeloma and 113 patients with lymphoma diagnosed treated in Sichuan people's Hospital from January to December 2019 were selected and the total IgG and subclass IgG1,IgG2,IgG3 and IgG4,and some parameters of peripheral blood routine in patients were retrospective analyzed.Independent sample t test or nonparametric Mann Whitney U test were used for comparison between the groups.The relationship between IgG subclass and blood cell parameters was analyzed by correlation analysis.For the bivariate normal distribution data,Pearson correlation coefficient was calculated.For bivariate non normal distribution data,the Spearman correlation coefficient was calculated.Results:IgG1 and IgG2 were significantly higher in patients with multiple myeloma than in patients with lymphoma(P=0.001,0.000,respectively),but IgG3 and IgG4 were significantly lower than in patients with lymphoma(P=0.000,0.000,respectively),and there was no significant difference for total serum IgG between the two groups(P=0.717).The results showed that the IgG4 content of male patients with multiple myeloma and lymphoma was significantly higher than that of female patients(Z=-3.191,P=0.001);the age,M%,E%of the MM patients were significantly higher than those of the patients with lymphoma(P=0.000,0.005,0.019),but WBC,RBC,Hb were significantly lower than those of the patients with lymphoma(P=0.013,0.000,respectively).The results of Spearman correlation analysis showed that RBC and Hb were decreased with the increasing of IgG and IgG1 in the MM patients(r=0.254,-0.272,-0.248 and-0.289,P=0.004,0.002,0.005 and 0.001).With the increasing of IgG4 in the serum of patients with lymphoma,RBC and Hb showed the trendy of decreased(r=-0.240 and,-0.251,respectively,P=0.010,0.007).Conclusion:The detection of IgG subclass and the correlation between IgG subclass and blood cell parameters are great value in the diagnosis and pathogenesis of multiple myeloma and lymphoma.