过敏性紫癜患者中IL-27对Th17细胞的影响

Effects of IL-27 on Th17 Cells in Patients with Henoch-Schonlein Purpura

目的:探讨过敏性紫癜(HSP)患者中IL-27对Th17细胞的影响,进一步探索HSP的发病机制。方法:选取本院2019年4月至2019年7月收治的过敏性紫癜患者50例为HSP组,同期行健康体检的30例志愿者作为对照组,采用流式细胞术(FCM)检测HSP组和健康对照组外周血中Th17细胞比例;选取27例HSP患者,用外源性rhIL-27与入选者外周血单个核淋巴细胞(PBMC)共培养,流式细胞术检测Th17细胞比例。结果:HSP患者急性期血清外周血中Th17细胞比例为(1.57±0.54)%,明显高于对照组的(0.86±0.40)%(t=-6.298,P<0.001),与rhIL-27培养后Th17细胞的比例明显下降(1.39%±0.52%vs 0.98%±0.44%)(P<0.05)。结论:HSP患者中IL-27可以降低Th17细胞水平,其可能参与HSP的致病过程并在疾病发展过程中发挥保护作用。

Objective: To investigate the effect of IL-27 on Th17 cells in patients with henoch-schonlein purpura(HSP) in order to further elucidate the pathogenesis.Methods: Fifty patients with HSP treated in our hospital from April 2019 to July 2019 were selected as HSP group,and 30 volunteers underwent physical examination at the same time were selected as control group.The proportion of Th17 cells in peripheral blood of HSP group and healthy control group was determined by flow cytometry(FCM).A total of 27 HSP patients were selected,and candidate peripheral blood mononuclear lymphocytes(PBMC) were co-cultured with exogenous rhIL-27,and the ratio of Th17 cells was detected by flow cytometry.Results: The proportion of Th17 cells in the peripheral blood of HSP patients with acute phase was(1.57 ± 0.54) %,which was significantly higher than that of the control group(0.86 ± 0.40) %(t =-6.298,P <0.001),and the proportion of Th17 cells was decreased significantly after rhIL-27 co-culture(1.39% ± 0.52% vs0.98% ± 0.44%)(P < 0.05).Conclusion: IL-27 can reduce the level of Th17 cells in patients with HSP,which may be involved in the pathogenic process of HSP and play a protective role in the development of the disease.