牛血清中6种外源性病毒的液相芯片检测方法建立

Establishment of Liquichip Detection Method of Six Kinds of Exogenous Viruses in Bovine Serum

为建立牛血清中6种外源性病毒(BVDV、BPV、BTV、REOV、BPyV、RABV)的液相芯片检测方法,根据6种病毒的高度保守区域设计引物和探针,通过杂交偶联检测,利用Luminex检测仪检测荧光信号,建立了牛血清中6种外源性病毒的快速检测方法。结果表明,该试验建立的检测体系具有良好的特异性和敏感性,其检测灵敏度可达50拷贝/反应。该研究初步建立了同时检测牛血清中6种外源性病毒的液相芯片检测方法,为牛血清的质量把关提供了技术支持。

In order to establish the liquichip detection method of 6 kinds of exogenous viruses(BVDV,BPV,BTV,REOV,BPyV,RABV)in bovine serum,on the basis of the specific primers and their corresponding oligonucleotide probes of 6 kinds of viruses were designed,synthesized and modified.Specific probe labeled with biotin was prepared and coupled with fluorescence-coded microspheres.Luminex detector was used to detect fluorescence signal,a quick detection method of 6 kinds of exogenous viruses in bovine serum was established.The results showed that this assay method displayed good specificity and sensitivity.The sensitivity test indicated the detection sensitivity could reach 50 copies.A liquichip detection method for six kinds of exogenous viruses in bovine serum was preliminarily established,which provided the technology supports for the quality control of bovine serum.