响应面法优化车前草多糖超声工艺及抗氧化研究

Optimization of Ultrasonic Technology of Polysaccharide of Plantago asiatica by Response Surface Methodology and Its Antioxidant Activity Study

[目的]通过响应面法优化车前草多糖的超声提取工艺,并评价其抗氧化能力。[方法]以车前草多糖(PLP)含量为评价指标,结合单因素试验和Box-Behnken响应面法对车前草多糖提取工艺进行优化,获取最优技术参数;此外,对车前草多糖进行·OH和O 2·-清除能力的考察以对其抗氧化活性进行评价。[结果]车前草多糖最佳超声提取工艺条件为提取温度61℃、超声时间68 min、料液比1∶34,在此条件下,PLP含量可达12.25%±0.18%(n=3),与理论值仅相差0.12%;此外,当车前草多糖浓度为1 mg/mL时,其对O 2·-和·OH的清除率分别为75.46%±0.54%和70.17%±0.94%。[结论]基于响应面法优化的车前草多糖超声提取工艺准确可行,可用于车前草多糖的提取;车前草多糖具有一定的抗氧化能力。

[Objective]To optimize the ultrasonic extraction process of polysaccharide of Plantago asiatica by response surface methodology and evaluate its antioxidant capacity.[Method]Taking the content of polysaccharide of Plantago asiatica as the evaluation index,combining single factor test and Box-Behnken response surface method to optimize the extraction process of Plantago asiatica polysaccharide to obtain the best technical parameters.In addition,the scavenging ability of Plantago asiatica polysaccharides on·OH and O 2·-free radicals was investigated to evaluate their antioxidant activity.[Result]The optimal ultrasonic extraction process conditions for polysaccharide of Plantago asiatica were:extraction temperature 61℃,ultrasonic time 68 min,and solid-liquid ratio 1∶34.Under this condition,the PLP content could reach 12.25%±0.18%(n=3),which was only 0.12%different from the theoretical value.In addition,when the polysaccharide of Plantago asiatica was 1 mg/mL,the removal rate of·OH and O 2·-free radicals were about 75.46%±0.54%and 70.17%±0.94%,respectively.[Conclusion]The ultrasonic extraction process of Plantago asiatica polysaccharide optimized based on response surface method is accurate and feasible,and can be used for the extraction of Plantago asiatica polysaccharide;Plantago asiatica polysaccharide has a certain antioxidant capacity.