miR-146a基因多态性与帕金森病的相关性分析

The relationship between miR-146a gene polymorphism and Parkinson’s disease

目的分析微小RNA-146a(miR-146a)基因单核苷酸多态性(SNP)与帕金森病(PD)的相关性。方法采用病例对照研究,选取2015年10月-2019年8月本院连续就诊和住院治疗的原发性PD患者446例(PD组),另匹配同期健康体检中心健康体检者450例为对照组,采用DNA PCR测序技术检测miR-146a rs2910146、rs5709329位点SNP;采用Logistic回归分析影响PD发生的危险因素。结果PD组与对照组miR-146a rs2910146、rs5709329位点基因型分布均符合哈代-温伯格(Hardy-Weinberg)平衡定律(P>0.05)。PD组与对照组性别、年龄、BMI、文化程度比较差异均无统计学意义(P>0.05)。与对照组比较,PD组rs2910146位点G等位基因、GG基因型频率显著降低(P<0.05)。PD组与对照组rs5709329位点等位基因频率、基因型分布比较,差异无统计学意义(P>0.05)。miR-146a rs2910146位点SNP与PD患者认知功能障碍、年龄有关(P<0.05),与性别、PD亚型、首发肢体无关(P>0.05)。携带GG基因型是PD发生的独立保护因素(P<0.05)。结论miR-146a rs2910146位点SNP与PD患者年龄、认知障碍有关,携带GG基因型可能降低PD发生风险。

Objective To analyze the relationship between single nucleotide polymorphism(SNP)of microRNA-146a(miR-146a)gene and Parkinson’s disease(PD).Methods In a case-control study,446 patients(PD group)with primary PD were selected from our hospital from October 2015 to August 2019,and 450 healthy people in the health examination center of the same period were selected as the control group,the SNP of rs2910146 and rs5709329 loci in miR-146a were detected by DNA PCR sequencing technology;and the risk factors of PD were analyzed by logistic regression.Results The genotypes of rs2910146 and rs5709329 in miR-146a in PD group and control group were consistent with Hardy-Weinberg equilibrium law(P>0.05).There was no significant difference in gender,age,BMI and education between PD group and control group(P>0.05).Compared with the control group,the G allele and GG genotype frequency of rs2910146 locus in PD group were significantly lower(P<0.05).There was no significant difference in allele frequency and genotype distribution of rs5709329 locus between PD group and control group(P>0.05).SNP at rs2910146 in miR-146a was related to cognitive dysfunction and age of PD patients(P<0.05),but not to gender,PD subtype and first-onset limb(P>0.05).Carrying GG genotype was an independent protective factor for PD(P<0.05).Conclusion SNP at rs2910146 locus in miR-146a is related to age and cognitive impairment in PD patients,and carrying GG genotype may reduce the risk of PD.