奥沙利铂通过调控P53、miR-34a抑制胃癌细胞的增殖

Oxaliplatin in inhibiting proliferation of gastric cancer cells by regulating P53 and miR-34a

目的:探究奥沙利铂调控P53、miR-34a表达及对胃癌细胞增殖的影响。方法:选取胃癌BGC-823细胞,并构建过表达miR-34a胃癌BGC-823细胞,检测加入奥沙利铂后BGC-823及过表达miR-34a组的细胞活力,并测定各组细胞内P53的mRNA及蛋白表达水平。结果:与对照组相比,加药12 h组与24 h组细胞活力下降(均为P<0.05),过表达miR-34a组细胞活力下降(t=3.030、P=0.013);24 h组P53 mRNA和蛋白表达水平高于对照组(t=2.796、P=0.049,t=5.508、P=0.002),过表达miR-34a组的P53 mRNA和蛋白表达高于对照组(t=4.775、P=0.009,t=3.704、P=0.010)。结论:奥沙利铂可能通过激活miR-34a,诱导上游的P53基因及蛋白表达,起到促进胃癌细胞BGC-823凋亡、减缓肿瘤的浸润与淋巴结及远处转移的作用。

Objective:To explore the mechanism of oxaliplatin in inhibiting the proliferation of gastric cancer cells through regulating P53 and miR-34a.Methods:BGC-823 cells of gastric cancer were selected and overexpressed miR-34a BGC-823 cells were constructed to detect the cell activity of BGC-823 and overexpressed 34a groups after oxaliplatin was added,and the expression levels of m RNA and protein of P53 in each group were determined.Results:The cell activity of the 12h group and the 24 h group decreased significantly(both P<0.0001),while that of the 34a group decreased significantly(P=0.0127).The expression levels of P53 mRNA and protein in the 24 h group were higher than those in the control group(P=0.0490;P=0.0015),and the expression of P53 mRNA and protein in the overexpressed miR-34a group was higher than that in the control group(P=0.0088;P=0.0100).Conclusion:Oxaliplatin may promote the apoptosis of gastric cancer cell BGC-823 and slow down tumor infiltration,lymph node and distant metastasis by activating miR-34a and inducing the expression of upstream P53 gene.