应用体细胞不亲和性试验和SSR方法综合鉴定闽南地区双孢蘑菇种质资源

Agaricus bisporus Germplasms in Southern Fujian Identified by Somatic Incompatibility Test and SSR

【目的】分析评价引进双孢蘑菇种质资源间的亲缘关系与遗传多样性,为闽南地区双孢蘑菇种质资源的鉴定、保藏与分子育种提供理论基础。【方法】结合体细胞不亲和性试验和SSR分子标记技术,对供试的9个双孢蘑菇菌株进行鉴定和遗传多样性分析。【结果】体细胞不亲和性试验结果表明,供试菌株之间拮抗作用较明显,根据拮抗反应的有无,供试菌株分为5组。SSR结果表明,当相似性系数达到0.76水平时,供试菌株聚成4个类群,第1类群包括HK;第2类群包括901和A15;第3类群包括福蘑52;第4类群包括As2796、W192、W2000、福蘑58、福蘑38。【结论】拮抗反应与SSR分子标记2种方法的结果基本一致。综合这2种方法,推测菌株HK与其他供试菌株亲缘关系较远,尤其与供试的其他国内菌株存在较大差异,可以作为一个育种亲本材料。

【Objective】Genetic relationship and diversity of Agaricus bisporus germplasms recently introduced to southern Fujian were studied to facilitate the identification,preservation,and breeding of the mushroom resource.【Method】Both somatic incompatibility test and SSR molecular marker technology were used to determine the genetic diversity of 9 varieties of A.bisporus.【Result】Somatic incompatibilities of the germplasms allowed the separation of 5 distinct groups by the presence or absence of antagonistic effect among them.Utilizing a similarity coefficient of 0.77,SSR analysis clustered the germplasms into Group 1 that consisted of HK,Group 2 that included 901 and A15,Group 3 that had Fumo 52,and Group 4 that comprised of As2796,W192,W2000,Fumo 58,and Fumo 38.【Conclusion】From the results obtained by the somatic incompatibility test and SSR molecular marker technology,HK appeared to be remotely related to the other 8 germplasms,especially the domestic varieties.Hence,it could be a potential candidate for breeding purpose.