重组猪干扰素α对急性病毒性腹泻仔猪肾脏损伤的保护作用研究

Protective Effect of Recombinant Porcine Interferon on Renal Injury of Piglets with Acute Viral Diarrhea

为研究重组猪干扰素α(rPoIFNα)在人工感染仔猪病毒性腹泻模型上对肾脏损伤的保护作用,选取10日龄健康三元哺乳仔猪15头,随机分为rPoIFNα治疗组(使用剂量100万单位/头)、猪流行性腹泻病毒(PEDV)感染对照组和正常对照组,每组5头仔猪。rPoIFNα治疗组和感染对照组仔猪经口饲喂PEDV悬液4 mL(接种量为4.0×105.5 TCID50/头),正常对照组仔猪同步饲喂4 mL生理盐水,建立PEDV致哺乳仔猪病毒性腹泻模型。模型建立结果显示,攻毒后72 h,感染对照组仔猪全部出现腹泻、脱水等典型病毒性腹泻症状,仔猪小肠及肾脏病料组织的PEDV核酸检测为阳性。同时发现,该组仔猪血肌酐水平显著升高(P<0.05),尿比重较其他两组偏高,并检测出颗粒管型和尿蛋白。镜下HE染色病理学观察到肾间质组织中出现嗜酸性粒细胞,提示超敏反应参与对肾脏的损伤。rPoIFNα治疗组仔猪症状轻微且易恢复,PEDV核酸检测阳性率仅为30%。仔猪相关血液及病料组织样本检测结果显示,rPoIFNα对PEDV感染仔猪治疗有效率达到60%。试验表明,rPoIFNα可有效治疗PEDV感染引起的仔猪腹泻,同时对肾脏具有明显保护作用。

The aim of this study was to investigate the protective effect of recombinant porcine interferon α on kidney injury in piglets model with viral diarrhea.15 ten-day-old healthy ternary suckling piglets were randomly divided into rPoIFNα treatment group(1 million units per pig), porcine epidemic diarrhea virus(PEDV)infection control group and normal control group,5 piglets in each group. Piglets in the infection control group and rPoIFNα treatment group were orally fed with 4 mL of PEDV virus suspension(4.0×105.5TCID50 per pig) and the normal control group was fed with 4 mL normal saline at the same time. Establishment of porcine viral diarrhea model infected with PEDV. The results showed that 72 hours after virus infection, all the infected control group had typical diarrhea symptoms such as diarrhea and dehydration. The PEDV nucleic acid test of piglets in small intestine and kidney was positive. At the same time, it was found that the serum creatinine level of piglets was significantly increased(P<0.05), the urine specific gravity in the infection control group was higher than that in the other two groups, and there were granular tubules and proteinuria symptoms. Microscopically, eosinophilic granulocytes were observed in renal interstitial tissue by HE staining, indicating the renal injury caused by hypersensitivity. The symptoms of piglets in the rPoIFNα treatment group were mild and easily recovered, and the positive rate of PEDV nucleic acid detection is only 30%. The detection results of blood and pathological tissue samples from piglets showed that the effective rate of rPoIFNα on PEDV infected piglets was 60%. The results showed that rPoIFNα could effectively treat piglet diarrhea caused by PEDV infection, and had a significant protective effect on the kidney.