丁公藤对大鼠膝骨关节炎滑膜炎症及痛阈的影响

The Effect of Erycibe Obtusifolia Benth on the Synovial Inflammation and Threshold of Pain in KOA Rats

目的探讨丁公藤对大鼠膝骨关节炎(KOA)滑膜炎症及痛阈的影响。方法将30只SPF级SD大鼠,随机均分为空白组、模型组和丁公藤组。通过前交叉韧带切除术(ACLT)构建KOA模型,造模成功后,丁公藤组外敷丁公藤28 d。于造模前,造模后第7、14、21、28、35、42天检测各组大鼠冷痛敏阈值和机械痛敏阈值,末次给药后腹主动脉采血,提取各组背根神经节、滑膜组织,HE染色观察滑膜炎症;Western blot和PCR检测滑膜IL-1β、TNF-α,背根神经节TRPA1、TRPV4的蛋白和基因表达量。结果HE染色显示,与模型组比较,丁公藤组炎性细胞浸润明显减少,细胞排列较为规则;模型组冷痛敏阈值、机械痛敏阈值与空白组相比均显著降低(P<0.01),丁公藤组较模型组均显著升高(P<0.01);模型组IL-1β、TNF-α、TRPA1和TRPV4的蛋白和基因水平较空白组增加(P<0.05~0.01),丁公藤组较模型组表达下调(P<0.05~0.01)。结论丁公藤能抑制滑膜炎症,缓解KOA大鼠冷刺激痛和机械刺激痛。

OBJECTIVE To investigate the effect of Erycibe obtusifolia Benthon on synovial inflammation and threshold of pain in KOA rats.METHODS 30 SPF SD rats were randomly divided into normal group,model group and Erycibe obtusifolia Benthon group.The KOA model was constructed by ACLT.After modeling,Erycibe obtusifolia Benthon was applied in Erycibe obtusifolia Benthon group for 28 days.The cold pain threshold and mechanical withdrawal threshold in each group were measured before modeling and on the 7th,14th,21th,28th,35th and 42th day after modeling.After the final administration,blood was collected from the abdominal aorta,DRG and synovial tissues of each group were extracted.Synovitis was observed by HE staining;then IL-1β,TNF-α,TRPA1 and TRPV4 protein and gene expression in synovial tissues and DRG were detected by Western blot and PCR.RESULTS HE staining showed that inflammatory cell infiltration in the Erycibe obtusifolia Benthon group significantly reduced compared with the model group,and the arrangement was relatively regular.The cold pain threshold and the mechanical withdrawal threshold in the model group were significantly lower than those of the normal group(P<0.01),and the thresholds in the Erycibe obtusifolia Benthon group were significantly higher than those in the model group(P<0.01).The levels of IL-1β,TNF-α,TRPA1 and TRPV4 protein and gene expressed in the model group were significantly higher than those in the normal group(P<0.05,P<0.01).The levels of IL-1β,TNF-α,TRPA1 and TRPV4 protein and gene expression in the Erycibe obtusifolia Benthon group were significantly lower than those in the model group(P<0.05,P<0.01).CONCLUSION Erycibe obtusifolia Benthon can inhibit synovitis inflammation and relieve the cold pain threshold and mechanical withdrawal threshold in KOA rats.