半夏厚朴汤对顺铂所致水貂呕吐模型的作用及机制研究

Effect and Mechanism of Banxia Houpu Tang on Mink Vomiting Model Induced by Cisplatin

目的探讨半夏厚朴汤(BXHPT)对顺铂所致水貂呕吐模型的治疗作用以及对P物质(SP)/NK1R信号的影响。方法36只水貂随机分为正常对照组,模型组,昂丹司琼组,BXHPT低、中、高剂量组(200、400、800 mg/kg),各组分别给予相应的药物连续7 d,后采用顺铂腹腔注射构建呕吐模型,测定水貂的干呕和呕吐次数,ELISA法检测水貂大脑最后区SP的含量,Western blot法测定大脑最后区NK1R、p-ERK1/2、ERK1/2的蛋白水平。结果模型组水貂干呕、呕吐次数,大脑最后区SP水平,NK1R,p-ERK1/2蛋白水平均明显高于正常对照组(P<0.05)。而BXHPT组水貂干呕、呕吐次数,大脑最后区SP水平,NK1R,p-ERK1/2蛋白水平均明显低于模型组(P<0.05)。结论BXHPT可以治疗顺铂导致的水貂的呕吐,其机制可能与降低水貂大脑最后区SP/NK1R信号活化有关。

OBJECTIVE To investigate the effect of Banxia Houpu Tang(BXHPT)on the model of mink vomiting induced by cisplatin and substance P/NK1R pathway.METHODS 36 minks were randomly divided into normal control group,model group,ondansetron group,BXHPT low,medium and high dose group(200,400,800 mg/kg).Each group was given corresponding drugs,respectively,for seven days.Then intraperitoneal injection of cisplatin was used to construct vomit model,the times of retching and vomit were measured,the content of substance P in the last area of mink brain were measured by ELISA,the protein levels of NK1R,p-ERK1/2 and ERK1/2 in the last area of the brain were measured by Western blot.RESULTS The levels of retching,vomit times,substance P,NK1R and p-ERK1/2 protein in the last area of brain in the model group were significantly higher than those in the normal control group(P<0.05).In BXHPT group,the retch,vomit times,substance P level,NK1R and p-ERK1/2 protein levels were significantly lower than those in model group(P<0.05).CONCLUSION BXHPT can treat the vomiting of mink caused by cisplatin,and the mechanism may be related to the decrease of substance P/NK1R signal activation in the last brain area of minks.