摘要
目的:建立一种太子参环肽单体的分离制备方法。方法:采用硅胶柱层析分离制备太子参环肽粗分物,经制备级高效液相色谱(HPLC)纯化,使用质谱(MS)、核磁共振波谱(NMR)及二维核磁共振波谱对其结构进行表征。结果:通过质谱、核磁共振波谱法确定了所得环肽为太子参环肽Pseudostellaria A (PA)。结论:建立一种太子参环肽PA的分离制备方法,提取率为32. 5μg/g,纯度为95. 16%。
Objective To establish a method for the separation and preparation of Pseudostellaria heterophylla cyclic peptide monomer.Methods The crude cyclic peptide of Pseudostellaria heterophylla was separated and prepared by silica gel column chromatography,purified by preparative High Performance Liquid Chromatography(HPLC),and its structure was characterized by Mass spectrometry(MS),Nuclear Magnetic Resonance spectroscopy(NMR)and two-dimensional nuclear magnetic resonance spectroscopy.Results The obtained cyclic peptide was determined to be Pseudostellaria A(PA)by mass spectrometry and nuclear magnetic resonance spectroscopy.Conclusion A method for the separation and preparation of Pseudostellaria heterophylla cyclic peptide PA has been established,the extraction rate is 32.5μg/g,and the purity is 95.16%.
作者
贺潇宇
赵立
魏丹
蒋静怡
庞文生
胡娟
HE Xiaoyu;ZHAO Li;WEI Dan;JIANG Jingyi;PANG Wensheng;HU Juan(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Second People s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China)
出处
《中国民族民间医药》
2020年第22期47-52,共6页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
国家自然科学基金项目(81673575)
国家重点研发计划“中医药现代化研究”重点专项(2019YFC1710504)。
关键词
太子参
环肽
核磁共振波谱法
质谱法
柱层析
Pseudostellaria heterophylla
Cyclic Peptide
Nuclear Magnetic Resonance Spectroscopy
Mass Spectrometry
Column Chromatography
