葛根素联用沙格列汀在高糖环境下对人脐静脉内皮细胞的影响

Effects of puerarin with saxagliptin on the function of human umbilical vein endothelial cells under high glucose condition

目的:观察葛根素联用沙格列汀在高糖环境下对人脐静脉内皮细胞(HUVEC)的影响,并探讨其可能机制。方法:选取处于对数生长期的HUVEC细胞,分为空白对照组、高糖模型组、葛根素给药组、沙格列汀给药组和葛根素联用沙格列汀给药组,观察药物对生长状况的影响,采用MTT法观察药物浓度作用效应;AnnexinⅤ-FITC/PI双染检测细胞凋亡率,RT-PCR检测P-选择素和NF-κB p65mRNA的表达。结果:葛根素联用沙格列汀能降低高糖诱导的HUVEC凋亡率,降低胞内P-选择素和NF-κB p65的表达。结论:葛根素联用沙格列汀对高糖环境下内皮细胞损伤具有一定的保护作用,并且可有效抑制高糖条件下诱导的内皮细胞损伤后P-选择素的活化;其保护效应可能通过NF-κB信号通路发挥作用。

Objective:To investigate the effects of puerarin combined with saxagliptin on and potential mechanisms in human umbilical vein endothelial cells(HUVECs)under the condition of high glucose.Methods:The cells in logarithmic growth stage were divided into groups of blank control,high sugar,single puerarin and saxagliptin,and combined use of puerarin and saxagliptin to observe theiReffects on cell growth in each group.MTT assay was performed to measure the cell proliferation to different drug concentration.Apoptosis rate of cells was detected by AnnexinⅤ-FITC/PI staining,and the mRNA expression of P-selectin and NF-κB p65 was examined by Q-PCR.Results:Puerarin combined with saxagliptin inhibited the cell viability and cell apoptosis induced by high glucose,and reduced mRNA expression of P-selectin and NF-κB p65.Conclusion:Puerarin combined with saxagliptin can be certain protective effect on the injury of HUVECs,and induce activation of P-selectin after damage of HUVECs from high glucose condition.The potential mechanism of such protective effect must be involved in NF-κB signaling pathway.