Ack1和MMP-14在涎腺腺样囊性癌组织中的表达与意义

Expression and significance of Ack1/MMP-14 in salivary adenoid cystic carcinoma

目的:检测Ack1、MMP-14在涎腺腺样囊性癌(SACC)组织中的表达水平,分析两者与SACC发生和发展之间有无相关性。方法:以免疫组化SABC法分别检测Ack1和MMP-14在49例SACC组织、24例涎腺正常组织中的表达水平。结果:在SACC组织、涎腺正常组织中,Ack1的阳性细胞表达率分别为63.3%、33.3%(P<0.05);MMP-14的阳性细胞表达率分别为67.3%、37.5%(P<0.05);在SACC组织中,Ack1及MMP-14的表达水平与组织学类型、临床分期、肿瘤细胞是否会侵犯到颈部淋巴结以及是否转移至周围神经有关(P<0.05),但与患者性别和年龄无关(P>0.05);且两者在SACC组织中的表达呈正相关(R=0.372,P=0.009)。结论:Ack1和MMP-14与SACC的病情发展相关。

Objective:To determine the expression of activated Cdc42-associated tyrosine kinase 1(Ack1)and matrix metalloproteinase-14(MMP-14)in salivary adenoid cystic carcinoma(SACC)tissues,and analyze the correlation between Ack1 and MMP-14 in the development and progression of SACC.Methods:Immunohistochemistry was used to determine both Ack1 and MMP-14 expressed in the tissues of 49 cases of SACC and normal salivary tissues from 24 subjets.Results:The positive rate of Ack1 and MMP-14 was 63.3%and 33.3%,67.3%and 37.5%,respectively in SACC tissues and in normal salivary tissues(both P<0.05).The expression of Ack1 and MMP-14 in SACC was closely associated with tumor histological grade,clinical stage,cervical lymph node invasion and metastasis in peripheral nerve(all P<0.05),yet not involved in patient’s gender and age at diagnosis(both P>0.05).Expression of Ack1 and MMP-14 was positively correlated in SACC(R=0.372,P=0.009).Conclusion:Present findings suggest that Ack1 and MMP-14 play a vital role in the progression of SACC.