副猪嗜血杆菌河南分离株的优势血清型和毒力基因研究

Reserch on Dominant Serotypes and Virulence Genes of Haemophilus Parasuis Isolates from Henan Province

为了解河南省副猪嗜血杆菌(Haemophilus parasuis,HPS)的血清型和毒力基因的流行与分布情况,对2017-2018年河南地区分离到的46株HPS,利用PCR反应鉴定血清型与毒力基因。结果显示,46株HPS共检测出7种血清型(2、4、5/12、6、7、11、13),血清型5/12和4为优势血清型,最流行的血清型为5/12型(15/46,32.6%),其次是4型(12/46,26.1%)和7型(7/46,15.2%)。强毒株和中等毒力株含有大多数毒力基因,无毒力菌株一般只含有vta1、ompP2、nanH和espP2基因。可见,毒力基因与血清型有很强的相关性。

In order to investigate the prevalence and distribution of serotypes and virulence genes of Haemophilus parasuis(HPS)in Henan Province,PCR reactions were used to identify the serotypes and virulence genes of 46 HPS strains isolated in Henan Province from 2017 to 2018.The results showed that 46 strains of HPS detected a total of 7 serotypes(2,4,5/12,6,7,11,13),serotypes 5/12 and 4 were dominant serotypes,and the most popular serotype was 5/12 serotype(15/46,32.6%),followed by serotype 4(12/46,26.1%)and serotype 7(7/46,15.2%).Most virulence genes were found in virulent strains and moderately virulent strains,and non-virulent strains generally only contained vta1,ompP2,nanH and espP2 genes.It can be seen that virulence genes have a strong correlation with serotypes.