粗皮桉芽器官离体快繁研究

Study on Rapid in Vitro Propagation of Eucalyptus pellita Buds

为提高粗皮桉组培成活率,用粗皮桉优树伐桩萌芽的茎段作外植体,改良MS作基础培养基,以芽器官直接离体培养成苗的方式建立无菌培养材料,经继代增殖培养,诱导生根,瓶苗移栽等环节,建立粗皮桉组培快繁体系。结果表明:继代苗增殖系数3.5~4.2,瓶苗生根率95%,移栽成活率92.5%,能够大规模商业化育苗。

In order to improve the survival rate of tissue cultured Eucalyptus pellita,stem segments collected from coppice sprouts of E.pellita were used as explants for culturing with a basic MS culture medium.Aseptic culture material was established by direct in vitro culture of bud organs.Subsequent subculture to proliferate available shoots followed by inducement of rooting and then transplanting of rooted plantlets enabled rapid propagation of E.pellita.The results showed that the multiplication coefficient of secondary(sub-cultured)seedlings was 3.5~4.2,the rooting rate of the in vitro explants was 95%,and then survival rate after transplanting was 92.5%.Thus,the methods employed can be used for large-scale commercial propagation.