miR-21在原发性肝癌组织中的表达及对细胞生物学行为的影响

Expression of miR-21 in Primary Hepatocellular Carcinoma Tissues and the Influence on Cell Biological Behavior

目的探讨miR-21在原发性肝癌组织中的表达及对细胞生物学行为的影响。方法选取2014年7月至2019年3月我院收治的原发性肝癌患者100例,分离其肝癌组织和癌旁正常组织,选择肝癌组织HepG2细胞,按转染方式分为抑制组和增强组。抑制组采用Has-miR-21 inhibitor (100 nm/L)和Lipofectamine 2000进行转染,增强组采用Hsa-miR-21 mimics (50 nm/L)和Lipofectamine 2000进行转染。采用实时荧光定量PCR (qPCR)法检测血清MAP2K3和miR-21的表达水平,MTT增殖实验检测细胞增殖数。结果 qPCR检测结果显示,癌旁正常组织、肝癌组织的miR-21表达量分别为67.86±0.59、 89.63±4.42;MAP2K3表达量分别为5853.61±271.85、 1396.78±97.36,比较有统计学差异(P <0.05);抑制组的增殖细胞数显著低于增强组(P <0.05)。结论在原发性肝癌组织中miR-21呈过表达,可通过抑制miR-21表达,进而靶向调节MAP2K3表达水平,降低肝癌细胞的增殖、迁移和侵袭。

Objective To explore the expression of miR-21 in primary hepatocellular carcinoma tissues and the influence on cell biological behavior.Methods 100 patients with primary hepatocellular carcinoma admitted to our hospital from July 2014 to March 2019 were selected,and the hepatocellular carcinoma tissues and paracancerous tissues were isolated.The HepG2 cells were selected and divided into inhibition group and enhancement group according to the transfection method.The inhibition group was transfected with Has-miR-21 inhibitor(100 nm/L)and Lipofectamine 2000,while the enhancement group was transfected with Hsa-miR-21 mimics(50 nm/L)and Lipofectamine 2000.Real-time fluorescence quantitative PCR(qPCR)method was used to detect the expression levels of serum MAP2K3 and miR-21.The MTT proliferation experiment was used to detect the number of proliferating cells.Results qPCR results showed that the expression levels of miR-21 in paracancerous tissues and hepatocellular carcinoma tissues were 67.86±0.59 and 89.63±4.42;the expression levels of MAP2K3 in paracancerous tissues and hepatocellular carcinoma tissues were 5853.61±271.85 and 1396.78±97.36(P<0.05).The number of proliferating cells in the inhibition group was significantly lower than that in the enhancement group(P<0.05).Conclusions miR-21 is overexpressed in primary hepatocellular carcinoma tissues,which can inhibit the expression of miR-21 and further regulate the expression level of MAP2K3,and reduce the proliferation,migration and invasion of liver cancer cells.