灰树花液体种子培养基的优化

Optimization of Liquid Seed Medium for Grifola frondosa

本文采用摇瓶培养法通过单因素和正交试验对灰树花液体种子的培养基进行了优化。单因素试验结果表明,大豆粉和麦芽糖是最适的氮源和碳源,MgSO_4是最适的无机盐。正交实验结果表明,灰树花的液体种子培养基的最优组合为:20%马铃薯,0.75%大豆粉,5%麦芽糖,0.1%KH_2PO_4,0.05%MgSO_4,0.001%VB1。采用优化后的培养基,在接种量为5%,温度28℃,转速为150 r/min的培养条件下振荡培养10 d,菌丝体干重超过1.322 8 g/100mL。

In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder and maltose were the optimal nitrogen and carbon sources,and MgSO4 was the optimal inorganic salt.The orthogonal experiment results showed that the optimal combination of liquid seed culture medium for Grifola frondosa was:20%potato,0.75%soybean powder,5%maltose,0.1%KH2PO4,0.05%MgSO4,0.001%VB1.Using the optimized medium,shaking culture for 10 d under the inoculum amount of 5%,the temperature of 28℃,and the rotation speed of 150 r/min,the dry weight of mycelium exceeded 1.3228 g/100mL.