摘要
目的:探讨高尔基体磷蛋白3(GOLPH3)、NOD样受体蛋白3(NLRP3)在胆囊癌中的表达及临床意义。方法:收集中国人民解放军联勤保障部队第904医院2014年1月至2019年1月行胆囊根治术的63例胆囊癌患者手术标本和临床资料,纳入胆囊癌组,男性21例,女性42例,平均年龄62.5岁。另取同期胆囊轻中度不典型增生的30例患者标本,纳入癌前病变组,男性9例,女性21例,平均年龄62.4岁。取因肝脏创伤或巨大肝血管瘤行外科手术的20例患者切除的正常胆囊标本,纳入正常组,男性7例,女性13例,平均年龄61.9岁。免疫组化检测各组胆囊组织GOLPH3、NLRP3、Ki-67的表达,并计数阳性率。log-rank检验及Cox回归分析胆囊癌患者GOLPH3、NLRP3表达与生存预后的关系。结果:GOLPH3、NLRP3、Ki-67在胆囊癌组、癌前病变组、正常组表达依次降低。胆囊癌组织中GOLPH3、NLRP3表达阳性率均与Ki-67表达阳性率呈正相关( r=0.972和 r=0.969,均 P<0.05)。多因素分析,GOLPH3阳性( HR=4.891,95% CI:1.776~13.470)、NLRP3阳性( HR=3.006,95% CI:1.273~7.099)是胆囊癌患者术后生存的独立危险因素(均 P<0.05)。 结论:GOLPH3和NLRP3蛋白在胆囊癌组织中高表达,GOLPH3和NLRP3阳性是胆囊癌患者术后生存的独立危险因素。
Objective To investigate the expression and clinical significance of Golgi phosphoprotein 3(GOLPH3)and NOD-like receptor protein 3(NLRP3)in gallbladder carcinoma(GBC).Methods Surgical specimens and clinical data were collected from 63 patients with GBC who underwent radical cholecystectomy in the 904th Hospital of Joint Logistic Support Force of PLA from January 2014 to January 2019.In the GBC group,there were 21 males and 42 females,with an average age of 62.5 years.For 30 patients with mild to moderate atypical hyperplasia of gallbladder during the same period were included in the precancerous lesion group,including 9 males and 21 females,with an average age of 62.4 years.Normal gallbladder specimens from 20 patients who underwent surgical resection due to liver trauma or giant hepatic hemangioma were collected and included in the normal group,including 7 males and 13 females,with an average age of 61.9 years.The expressions of GOLPH3,NLRP3,Ki-67 were detected by immunohistochemistry.Log-rank test and Cox regression were adopted to analyze the GOLPH3 and NLRP3 expression and survival prognosis of gallbladder cancer patients.Results Expression of GOLPH3 and NLRP3 in the tumor group,precancerous lesion group and normal group was decreased separately.In GBC tissues,the level of GOLPH3 and NLRP3 was positively correlated with the Ki-67 expression(r=0.972 and r=0.969,both P<0.05).Multivariate analysis showed that high level of GOLPH3(HR=4.891,95%CI:1.776-13.470)and NLRP3(HR=3.006,95%CI:1.273-7.099)was an independent risk factor for predicting the postoperative survival of patients with GBC(both P<0.05).Conclusion GOLPH3 and NLRP3 are highly expressed in GBC tissues,and high expression of GOLPH3 and NLRP3 is an independent risk factor for postoperative survival in patients with GBC.
作者
朱振成
罗昆仑
刘洪
方征
李福利
柏杨
刘伟伟
单留群
赵海滨
Zhu Zhencheng;Luo Kunlun;Liu Hong;Fang Zheng;Li Fuli;Bai Yang;Liu Weiwei;Shan Liuqun;Zhao Haibin(Department of Hepatobiliary Surgery,the 904th Hospital of Joint Logistic Support Force of PLA(Wuxi Clinical School of Anhui Medical University),Wuxi 214044,Jiangsu Province,China;Department of Pathology,the 904th Hospital of Joint Logistic Support Force of PLA(Wuxi Clinical School of Anhui Medical University),Wuxi 214044,Jiangsu Province,China)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2020年第11期846-849,共4页
Chinese Journal of Hepatobiliary Surgery
基金
南京军区医药卫生科研基金(14D05)。
关键词
胆囊肿瘤
高尔基体磷蛋白3
NOD样受体蛋白3
预后
影响因素
Gallbladder neoplasms
Golgi phosphoprotein 3
NOD-like receptor protein 3
Prognosis
Influencing factors