小麦水通道蛋白基因TaTIP1-1c-4BL的克隆与表达分析

Cloning and Expression Analysis of Wheat Aquaporin Gene TaTIP1-1c-4BL

为探究TaTIP1-1c-4BL基因在小麦响应缺水胁迫中的作用,采用PCR扩增的方法从小麦‘科农199’cDNA中获得TaTIP1-1c-4BL基因。生物信息学分析显示该基因编码区全长753 bp,共编码250个氨基酸。TaTIP1-1c-4BL蛋白具有6个跨膜域,为非分泌型、弱酸性、稳定型、疏水性蛋白。系统发育分析显示‘科农199’与来自粗山羊草和大麦的水通道蛋白亲缘关系较近。表达分析显示TaTIP1-1c-4BL基因在小麦根、茎、叶、籽粒及颖壳中均有表达,在茎中表达量最高。在PEG胁迫下,该基因的表达量下调,表明该基因在小麦应对干旱胁迫过程中具有负调控作用。在NaCl、ABA及H 2O 2胁迫下,表达量先下调后上调,推测该基因参与了某些抗逆信号的传导。

To investigate the role of wheat TaTIP1-1c-4BL gene in response to water stress.TaTIP1-1c-4BL was obtained from cDNA of wheat cultivar‘Kenong 199’by PCR in this study.The bioinformatics analysis showed that the coding region of the gene was 753 bp in length and encoded a total of 250 amino acids.TaTIP1-1c-4BL protein had 6 transmembrane domains and was a non-secreting,weakly acidic,stable,hydrophobic protein.Phylogenetic analysis showed that TaTIP1-1c-4BL was closely related to aquaporins from Aegilops chinensis and Hordeum vulgare.The expression analysis showed that TaTIP1-1c-4BL gene was expressed in roots,stems,leaves,grains and glumes,and the highest expression level was in stems.Under PEG stress,the expression of this gene was down-regulated.Under NaCl,ABA and H 2O 2 stresses,the expression was first down-regulated and then up-regulated,so we speculated this gene was involved in the transmission of some stress-resistant signals.