白桦AP2/ERF家族基因的生物信息学分析

Bioinformatic Analysis on AP2/ERF Genes from Betula platyphylla

从白桦转录组数据库中筛选出45条AP2/ERF家族蛋白,对其蛋白序列进行生物信息学分析和系统进化分析。结果表明,白桦AP2/ERF蛋白除了分为AP2、ERF、RAV和soloist家族外,还有2条蛋白不属于这些家族;不同组别白桦AP2/ERF蛋白在理化性质上存在差异;白桦AP2/ERF蛋白都含有磷酸化位点,但含有磷酸化位点的数量和种类上都有差异;白桦AP2/ERF蛋白大都定位在细胞核里,它们主要在细胞核中对下游基因进行转录调控;同一家族蛋白具有相同的三级结构及相同的motif,不同家族之间则存在差异。分析结果为白桦ERF基因功能研究提供参考。

In this study,we identified 45 ERF genes from transcriptome database of Betula platyphylla,and these ERF proteins were used for bioinformatic analysis and phylogenetic analysis.The study showed that,AP2/ERF proteins of B.platyphylla could be devided into AP2,ERF,RAV and soloist family,except two proteins do not belong to these families;The Physical and chemical properties of different AP2/ERF groups proteins are different;All members have phosphorylation sites,but they are different in number and types;Most AP2/ERF proteins located in nucleus,suggesting that they control transcription of downstream genes mainly in nucleus;Members in same family have similar tertiary structure and motifs,and they are different to members in other families;These results provide a reference for function studies on B.platyphylla AP2/ERF genes.