调控TGF-β1/Smad4通路对宫颈癌SiHa细胞自噬基因p62的影响

Effect of Regulating TGF-β1/Smad4 Pathway on Cervical Cancer Si Ha Cell Autophagy Gene p62

目的探究调控TGF-β1/Smad4通路对宫颈癌SiHa细胞自噬基因p62的影响。方法对数生长期的宫颈癌SiHa细胞加入不同浓度的TGF-β1和SIS3干预24h后收取细胞,分别检测在mRNA及蛋白水平上自噬基因p62表达量的变化。结果TGF-β1作用SiHa细胞24h后,随着浓度升高,p62 mRNA及蛋白的表达量逐渐降低(P<0.05)。SIS3作用SiHa细胞24h后,随着浓度升高,p62 mRNA及蛋白的表达量逐渐增高(P<0.05)。结论外源性的TGF-β1/Smad4通路激活剂TGF-β1可使宫颈癌SiHa细胞自噬蛋白p62 mRNA及蛋白的表达量降低,诱导宫颈癌SiHa细胞发生自噬;外源性的TGF-β1/Smad4通路抑制剂SIS3可使宫颈癌SiHa细胞自噬蛋白p62 mRNA及蛋白的表达量升高,抑制宫颈癌SiHa细胞发生自噬;且随TGF-β1和SIS3浓度的增加,p62表达量出现变化,具有一定的浓度依赖性。

Objective To investigate the effect of regulating TGF-β1/Smad4 pathway on cervical cancer SiHa cell autophagy gene p62.MethodsThe cervical cancer SiHa cells in the logarithmic growth phase were added with different concentrations of TGF-β1 and SIS3,and the cells were collected after 24 hours of intervention.The changes of the expression of autophagy gene p62 at the mRNA and protein levels were detected.ResultsAfter 24 hours of TGF-β1 treatment on SiHa cells,as the concentration increased,the expression of p62 mRNA and protein gradually decreased(P<0.05).After 24 hours of treatment of SiHa cells with SIS3,the expression of p62 mRNA and protein gradually increased with the increase of concentration(P<0.05).ConclusionThe exogenous TGF-β1/Smad4 pathway activator TGF-β1 can reduce the expression of autophagy protein p62 mRNA and protein in cervical cancer SiHa cells,induce autophagy in cervical cancer SiHa cells.Exogenous TGF-β1/Smad4 pathway inhibitor SIS3 can increase the expression of autophagy protein p62 mRNA and protein in cervical cancer SiHa cells,and inhibit autophagy in cervical cancer SiHa cells;and with the increase of TGF-β1 and SIS3 concentrations,p62 expression appears change with a certain concentration dependence.