rs2282679基因位点多态性与大学生维生素D缺乏相关

Relationship between rs2282679 gene locus polymorphism and vitamin D deficiency in college students

目的探讨大学生维生素D结合蛋白基因(GC)位点rs2282679多态性与血液维生素D水平之间的关系。方法从河北省某高校筛选符合条件的286名学生作为研究对象。采用液相色谱-质谱联用法定量分析血清25-羟化维生素D的水平,并用基因芯片进行rs2282679位点基因分型。采用条件Logistic回归模型分析rs2282679遗传突变对血清维生素D水平的影响。结果共有285名参与者完成了本研究,1名因数据不全而未纳入统计分析。在研究人群中,rs2282679基因位点A/C多态性的分布符合Hardy-Weinberg平衡。GC基因rs2282679位点的基因型与血液维生素D水平是否不足和缺乏呈显著相关性(P=0.031),等位基因A是维生素D不足和缺乏的风险因素。等位基因C相对于A的OR值是0.65,遗传方式是显性遗传(P=0.03)。结论维生素D结合蛋白基因rs2282679 A/C多态性与血清25-羟化维生素D不足和缺乏的风险相关,C为保护型碱基,A为危险型碱基,A等位基因可能是中国大学生维生素D不足和缺乏的危险因素。

OBJECTIVE To investigate the relationship between vitamin D binding protein gene rs2282679 A/C polymorphism with blood vitamin D levels.METHODS A total of 286 eligible subjects were selected from one university in Hebei Province.Serum 25-hydroxylated vitamin D levels were measured by liquid chromatography-mass spectrometry,and gene chip was used for genotyping of rs2282679 locus.RESULTS Statistical analysis was performed using R software.RESULTS A total of 285 participants in the study completed the experiment,and result indicated that the distribution of the rs2282679 locus A/C polymorphism in the participants was consistent with the Hardy-Weinberg equilibrium.The genotype of rs2282679 was significantly associated with serum vitamin D insufficiency and deficiency(P=0.031).Allele A was a risk factor for vitamin D insufficiency and deficiency.The OR value of allele C relative to A was 0.65,and the genetic pattern of allele C relative to A was dominant(P=0.03).CONCLUSION The vitamin D-binding protein gene rs2282679 was significantly associated with serum vitamin D insufficiency and deficiency in college students,and A-allele is a risk factor accounting for vitamin D insufficiency and deficiency in college students.