核转录因子与巨噬细胞移动抑制因子在染矽尘大鼠肺组织中的表达

Expression and significance of NF-κB and MIF in lung tissue of silicon-stained rats

目的探讨核转录因子(nuclear transcription factor,NF-κB)及巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)在染矽尘大鼠肺组织中的分布及表达。方法将64只10周~12周周龄的大鼠(清洁级)随机分为对照组和染矽尘组,各32只。在建模后第3天、7天、14天、28天每组随机处死8只大鼠,采用免疫组织化学方法检测大鼠肺组织中NF-κB和MIF的分布及表达。结果NF-κB p65阳性结果定位在细胞核和细胞浆,主要表达于支气管壁上皮细胞、巨噬细胞、中性粒细胞、肺间质细胞和肺泡上皮细胞中。染尘组NF-κB p65 IOD值在各时间点与对照组比较,差异均有统计学意义(P<0.05)。大鼠肺组织MIF免疫组化结果显示:MIF阳性表达细胞主要是肺泡上皮细胞、间质巨噬细胞和肺泡巨噬细胞,其中在部分毛细血管内皮中也有阳性表达,且血管壁平滑肌细胞中也有少量表达。染尘组MIF积分光密度值在各时间点与对照组比较,差异均有统计学意义(P<0.05)。结论染矽尘大鼠肺组织中NF-κB及MIF均呈阳性表达,在矽肺的早期诊断、治疗中具有一定的参考意义。

OBJECTIVE To investigate the distribution and expression of nuclear transcription factor(NF-κB)and macrophage migration inhibitory factor(MIF)in the lung tissue of rats exposed to silica.RESULTS Atotal of sixty-four adult rats aged 10 to 12 weeks(cleaning grade)were randomly divided into control group and silica dust group(32 rats each).At day 3,7,14,and 28 after modeling,8 rats were randomly sacrificed in each group.Immunohistochemistry was used to detect the distribution and expression of NF-B and MIF in lung tissue.RESULTS The positive result of NF-κB p65 were localized in the nucleus and cytoplasm,and were mainly expressed in bronchial wall epithelial cells,macrophages,neutrophils,lung mesenchymal cells,and alveolar epithelial cells.The IOD value of NF-κB p65 in the dust-exposed group was compared with the control group at each time point,and the difference was statistically significant(P<0.05).The result of MIF immunohistochemistry in rat lung tissue showed that:MIFpositive cells were mainly alveolar epithelial cells,mesenchymal macrophages and alveolar macrophages.Among them,positive expression was also found in part of the capillary endothelium,and in smooth muscle cells of the vessel wall There is also a small amount of expression.The MIF IOD value of the dust-exposed group was compared with the control group at each time point,and the difference was statistically significant(P<0.05).CONCLUSION Both NF-κB and MIF are positively expressed in the lung tissue of silica-exposed rats,which has certain reference significance in the early diagnosis and treatment of silicosis in the future.