浙江省部分水产品中8种维生素E异构体的含量

Eight vitamin E congeners in seafood and aquatic products in Zhejiang Province

目的分析浙江省鱼、虾、蟹等44种水产品中α-、β-、γ-、δ-生育酚(tocopherol,T)和α-、β-、γ-、δ-三烯生育酚(tocotrienol,T3)8种维生素E异构体的含量。方法采用碱液加热皂化,液液萃取净化富集,反相高效液相色谱-荧光检测器检测,外标法定量分析维生素E,开展各异构体的含量分析与比较。结果19种河鱼和14种海鱼中α-生育酚当量(α-tocopherol equivalent,α-TE)范围为0.10~4.01 mg/100 g可食部,所含维生素E异构体的种类有明显区别。河鱼中α-T、α-T3检出率为100%,γ-T、γ-T3检出率分别为31.58%和68.42%,而14种海鱼中α-T检出率为100%,α-T3、γ-T、γ-T3检出率分别为28.57%、28.57%、35.71%。在野生和养殖生长环境下,鳙鱼、白鲢鱼、汪刺鱼和大黄鱼中各异构体种类有差异,但含量差异无统计学意义。4种河虾蟹和7种海虾蟹中α-TE范围为0.31~14.27 mg/100 g可食部,均以α-T为主。母蟹中α-T含量略高于公蟹中,但差异无统计学意义,其他异构体种类及含量水平基本相同。海鱼中γ-T和α-T3有强相关性,河鱼中维生素E各异构体相关性较弱,虾蟹中维生素E各异构体有一定的相关性。结论不同淡水和海产品食物中维生素E含量水平差异较大,食用不同的水产品对机体的维生素E异构体和活性总量摄入有不同的影响。

OBJECTIVE Comparison and analysis ofα-,β-,γ-,δ-tocopherol(T)andα-,β-,γ-,δ-tocotrienol(T3)in 44 species of seafood and aquatic products is under processed to enrich the database of food composition in China and provide a scientific reference for dietary intake choice.METHODS Quantitative and correlation analysis of eight vitamin E isomers were based on external calibration method with reversed-phase high-performance liquid chromatography-fluorescence detector,after hot saponification with alkaline and liquid-liquid extraction.RESULTS The content ofα-tocopherol equivalent(α-TE)in seafood and aquatic products varied greatly(from 0.10 to 4.01 mg/100 g edible),as well as the isomer forms.Aspect of vitamin E forms in aquatic fish,detection rates ofα-T andα-T3 were both 100%,while the rates ofγ-T andγ-T3 were 31.58%and 68.42%,respectively.Aspect of vitamin E forms in sea fish,detection ratesα-T3,γ-T andγ-T3 were 28.57%,28.57%and 35.71%,respectively,while the rate ofα-T was 100%.The form of vitamin E isomers in fish was at some extent different when they raise up in wild and farming environment,whereas there was no significant different in content of isomers.For shrimp and crabs,the content ofα-TE was also various(from 0.31 to 14.27 mg/100 g edible),whereasα-T was the primary vitamin E form.And the content ofα-T in female crabs was a little higher than that in male crabs,without statistic difference.With respect to correlation analysis,there was a strong correlation betweenγ-T andα-T3 in sea fish,while weak correlation of isomers in aquatic fish and certain correlations of isomers in shrimp and crab.CONCLUSION The level of vitamin E content in seafood and aquatic products are quite different.Thus,it will bring in different effects on total activity and intake of vitamin E isomers by consumption of different species of seafood and aquatic products.