续断种子方对少精子症模型小鼠精子发生的影响

Effects of Xuduan Zhongzi prescription on spermatogenesis of mice with oligozoospermia

目的:研究续断种子方促进少精子症模型小鼠精子发生的机制。方法:45只雄性小鼠随机选取10只作为对照组,另外35只腹腔注射环磷酰胺建立少精子症模型,造模成功后按照随机数字表法分为对照组、续断种子方组、维生素E组各10只。续断种子方组和维生素E组分别按人体等效剂量给予相应药物灌胃,对照组和模型组给予生理盐水灌胃,8周后各组小鼠全部处死,用放免法检测睾酮水平;取一侧附睾做精子分析,另一侧睾丸切片行HE染色,计数生精上皮层次和间质细胞,TUNEL法检测生精细胞凋亡。结果:与对照组比较,模型组睾丸相对质量、精子质量、睾酮明显降低,生精细胞凋亡增多;与模型组比较,续断种子方组睾丸质量、精子质量、睾酮明显升高,生精细胞凋亡减少,差异有统计学意义。结论:续断种子方可通过改善精子发生的睾丸微环境,使间质细胞增多,分泌高水平睾酮,从而抑制生精上皮细胞凋亡,改善生精上皮环境,促进精子发生。

Objective:To study the mechanism of Xuduan Zhongzi Formula on promoting spermatogenesis in mice with oligospermia.Methods:10 male mice were selected in blank control group randomly.The other 35 male mice were treated by celiac injection of cyclophosphamide to make models.After successful modeling,the mice were randomly divided into blank group,Xuduan Zhongzi Formula group and Vitamin E group,10 male mice in each group.The treatment groups were respectively treated by intragastric administration of Xuduan Zhongzi Formula and Vitamin E.The blank and model group were treated by intragastric administration of saline.After 8 weeks’treatment,all the mice were sacrificed.The serum testosterone(T)level was determined by radioimmunology,and a suspension was made from the testis and epididymis of one side for sperm analysis,while the testis of the other side was sliced up and stained by HE method and TUNEL technique to detect the count of Leydig cells,the layers of spermatogenic epithelia and the apoptosis of spermatogenic cells.Results:Compared with the blank group,the weight of testis,sperm quality and testosterone in the model group were significantly reduced,and spermatogenic cell apoptosis was increased.Compared with the model group,the weight of testis,sperm quality,testosterone and spermatogenic cell apoptosis were significantly increased in the Xuduan Zhongzi Formula group,and the difference was statistically significant.Conclusion:Xuduan Zhongzi Formula can improve the micro-environment of spermatogenesis in the testicular,increase interstitial cells,secrete high levels of testosterone,thereby inhibit the apoptosis of spermatogenic epithelial cells and improve the spermatogenic epithelial environment to promote spermatogenesis.