骨髓Sca⁃1间充质干细胞对肺移植后急性肺损伤的影响

Effect of bone sca⁃1 marrow mesenchymal stem cells on acute lung injury after lung transplantation

目的评价骨髓Sca⁃1间充质干细胞对肺移植后急性肺损伤中的影响。方法成年SD大鼠24只,体质量250~300 g,采用随机数字表法分为3组。C组:空白对照组(n=8只),不做任何处理,仅做空白对照;M组(n=8只):模型组,SD大鼠行自体左肺原位移植术;B组(n=8只):模型+骨髓Sca⁃1间充质干细胞,SD大鼠行自体左肺原位移植术后,经尾静脉注射骨髓Sca⁃1间充质干细胞。术后48 h抽取大鼠动脉血,行动脉血气分析,然后处死大鼠,取肺部组织标本,进行含水率和病理学检测。采用ELISA法检测肺组织中H2O2、MDA、IL⁃6以及TNF⁃α水平,采用Western blot方法检测肺组织中Ⅱ型肺泡上皮细胞标志物SPB和气道黏膜上皮细胞标志物CC10的表达水平。结果与C组比较,M组大鼠动脉血中氧分压降低,肺组织出血、充血严重,炎性细胞浸润增多,肺水肿严重。肺组织中H2O2、MDA、IL⁃6以及TNF⁃α浓度升高(P<0.05);Ⅱ型肺泡上皮细胞标志物SPB和气道黏膜上皮细胞标志物CC10的表达水平降低。与M组比较,B组大鼠动脉血中氧分压升高,肺组织出血、充血减轻,炎性细胞浸润减少,肺水肿减轻。肺组织中H2O2、MDA、IL⁃6以及TNF⁃α浓度降低(P<0.05);Ⅱ型肺泡上皮细胞标志物SPB和气道黏膜上皮细胞标志物CC10的表达水平升高。结论骨髓Sca⁃1间充质干细胞可通过抑制炎性反应及氧化损伤、修复损伤的上皮细胞,改善肺功能,减轻肺移植后急性肺损伤。

Objective To investigate the effects of bone sca-1 marrow mesenchymal stem cells on acute lung injury after lung transplantation.Methods 24 adult Sprague-Dawley rats,weighing 250~300 g,were randomly allocated to 3 groups(n=8) using a random number table.Rats in group C without any treatment were blank control group(n=8).Rats in group M underwent autologous left lung orthotopic transplantation were model group(n=8).Rats in group B were injected with bone marrow sca-1 mesenchymal stem cells via the caudal vein after autologous left lung orthotopic transplantation.After 48 h,rat arterial blood was analyzed and lung tissue samples were taken for moisture content and pathological examination.ELISA was used to detect H2 O2,MDA,IL-6,and TNF-α levels in lung tissues.Western blot was used to detect the expression levels of type Ⅱ alveolar epithelial cell marker SPB and airway mucosal epithelial cell marker CC 10 in lung tissues.Results Compared with group C,PaO2 in the arterial blood was decreased,the bleeding and congestion of lung tissue were serious,the infiltration of inflammatory cells was increased,the pulmonary edema was serious,the concentrations of H2 O2,MDA,IL-6,and TNF-αwere significantly increased in lung tissues(P <0.05).Expression levels of type Ⅱ alveolar epithelial cell marker SPB and airway mucosal epithelial cell marker CC10 were down-regulated in group M.Compared with group M,PaO2 in the arterial blood was increased,the bleeding and congestion of lung tissue were mild,the infiltration of inflammatory cells was decreased,the pulmonary edema was mild,the concentrations of H2 O2,MDA,IL-6,and TNF-α were significantly decreased in lung tissues(P <0.05),the expression levels of type Ⅱ alveolar epithelial cell marker SPB and airway mucosal epithelial cell marker CC10 were up-regulated in group B.Conclusion Bone marrow sca-1 mesenchymal stem cells can alleviate acute lung injury after lung transplantation by inhibiting inflammatory response and oxidative damage,repairing damaged epithelial cells and improving lung function.