芦丁减轻全氟辛酸暴露致小鼠心肌损伤的作用研究

Effect of rutin on reducing myocardial injury induced by perfluorooctanoic acid exposure in mice

目的:探讨芦丁对全氟辛酸(PFOA)造成的小鼠心肌损伤的保护作用研究。方法:采用PFOA暴露建立小鼠心肌损伤模型,观察芦丁对PFOA造成小鼠心肌损伤模型体质量、心脏质量、心肌TG、TC含量和血清LDH活性、氧化应激指标变化(心肌MDA含量、GSH-PX和T-SOD活性)及心肌组织形态学变化。结果:各组小鼠体质量1~2 d比较差异无统计学意义(P>0.05);3~14 d各组小鼠体质量有差异,且PFOA组小鼠体质量低于PFOA+芦丁组(P<0.05);PFOA组和PFOA+芦丁组小鼠干预14 d心脏质量低于对照组,而PFOA+芦丁组高于PFOA组(P<0.05)。PFOA组小鼠14 d心肌TC含量及血清LDH活性高于对照组(P<0.05);PFOA+芦丁组小鼠14 d心肌TG含量及血清LDH活性低于PFOA组(P<0.05);而PFOA组与PFOA+芦丁组小鼠14 d心肌TC含量差异无统计学意义(P>0.05);PFOA组小鼠14 d心肌MDA含量高于对照组(P<0.05),而PFOA组与对照组小鼠14 d心肌T-SOD、GSH-PX活性差异无统计学意义(P>0.05);PFOA+芦丁组小鼠14 d心肌GSH-PX活性高于PFOA组,而PFOA+芦丁组与PFOA组在小鼠14 d时心肌MDA含量、GSH-PX活性差异无统计学意义(P>0.05)。结论:芦丁干预可以减轻PFOA暴露致小鼠心肌损伤作用。

Objective:To explore the protective effects of rutin on myocardial injury induced by perfluorooctanoic acid(PFOA)exposure in mice.Methods:PFOA was used to establish a model of myocardial injury in mice,and the effects of rutin on the body weight,heart weight,myocardial TG,TC content,serum LDH activity,and oxidative stress indicators(myocardial MDA content,GSH-PX and T-SOD activity)and myocardial morphology were investigated in mice.Results:There was no statistical significance in the weight of 1-2 d mice among all groups(P>0.05).The differences of in the weight of 3-14 d mice on day 8 to day 14 among all groups were statistically significant(P<0.05),and the weight of mice in PFOA group was lower than that in PFOA+rutin group,the heart weight of mice in PFOA group and PFOA+rutin group was lower than that in control group after 14 days of intervention,while the heart weight of mice in PFOA+rutin group was higher than that in PFOA group(P<0.05).After 14 days of intervention,the myocardial TC content and serum LDH activity in PFOA group were higher than those in control group(P<0.05),the myocardial TG content and serum LDH activity in PFOA+rutin group were lower than those in PFOA group(P<0.05),and the difference of the myocardial TC content between PFOA group and PFOA+rutin group was not statistically significant(P>0.05).After 14 days of inverention,the myocardial MDA content and in PFOA group was higher than those in control group(P<0.05),but there was no statistical significance in myocardial GSH-PX activity between PFOA group and the control group(P>0.05),the myocardial MDA content in PFOA+rutin group was lower than that in PFOA group,while the differences of the myocardial MDA content and GSH-PX activity between PFOA+rutin group and PFOA group were not statistically significant(P>0.05).Conclusions:Rutin intervention can ameliorate the myocardial injury induced by PFOA exposure in mice.