死产母猪血清中猪圆环病毒3型的研究

Study on Porcine Circovirus Type 3 in Serum of Stillborn Sows

为了搞清母猪血清中猪圆环病毒3型(PCV3)的存在是否与死产仔猪的发生有关,研究采用实时荧光定量PCR技术,对89头有死胎和无死胎的刚产母猪血清标本进行了PCV3感染率和血清中病毒DNA载量的检测。结果表明,在57份样品中都发现了PCV3基因组,基因组载量在每毫升血清中有10~200000个拷贝;在有或没有死产仔猪的母猪中,感染率或PCV3病毒载量没有显著差异。因此,母猪分娩时感染PCV3与死产仔猪的发生没有相关性。

To study whether the presence of PCV3 in the serum of sows is related to the occurrence of stillbirth,the real-time fluorescence quantitative PCR technique was used to detect the infection rate of PCV3 and the DNA load of serotype virus in 89 stillborn sows with and without stillbirth.The results showed that the PCV3 genome was found in 57 samples,and the genome load was 10-200000 copies in per milliliter of serum.There was no significant difference in infection rate or PCV3 viral load among sows with or without stillbirth.Therefore,there was no correlation between PCV3 infection and stillbirth in sows.