KIFC1基因对结直肠癌增殖与转移的影响

Effects of KIFC1 gene on the proliferation and metastasis of colorectal cancer

目的探讨KIFC1基因对结直肠癌增殖与转移的影响。方法免疫印迹检测KIFC1在结直肠癌与癌旁组织中的表达差异;KIFC1-siRNA转染人结直肠癌细胞,免疫印迹检测KIFC1蛋白表达情况;CCK-8实验检测KIFC1基因对结直肠癌细胞增殖影响;Transwell实验检测KIFC1基因对结直肠癌细胞转移能力的影响;免疫印迹检测EMT相关蛋白E-cadherin、N-cadherin、Vimentin表达变化。结果GEPIA数据库及免疫印迹结果显示KIFC1在结直肠癌组织中高表达;与对照组相比,KIFC1-siRNA可显著降低结直肠癌细胞KIFC1蛋白表达;CCK-8实验显示KIFC1-siRNA显著抑制结直肠癌细胞增殖能力;Transwell实验结果显示KIFC1-siRNA显著降低结直肠癌细胞转移能力;同时抑制KIFC1表达,可促进E-cadherin蛋白表达,抑制N-cadherin及Vimentin蛋白表达(P<0.05)。结论抑制KIFC1基因表达可以抑制结直肠癌细胞的增殖及转移能力。

Objective To investigate the effects of KIFC1 gene on the proliferation and metastasis of colorectal cancer.Methods Western blotting was used to detect the expression of KIFC1 in colorectal cancer and para-carcinoma tissues.Human colorectal cancer cells were transfected with KIFC1-siRNA,and the expression of KIFC1 was detected by Western blotting.CCK-8 assay was used to detect the effect of KIFC1 gene on the proliferation of colorectal cancer cells.Transwell assay was conducted to detect the effect of KIFC1 gene on the migration of colorectal cancer cells.Western blotting was used to detect the expression of EMT-related proteins,such as E-cadherin,N-cadherin and Vimentin.Results GEPIA database and immunoblotting results showed that KIFC1 was highly expressed in colorectal cancer tissue.Compared with the control group,KIFC1-siRNA could significantly reduce the expression of KIFC1 protein in colorectal cancer cells.CCK-8 assay showed that KIFC1-siRNA significantly inhibited the proliferation of colorectal cancer cells.Transwell assay showed that KIFC1-siRNA significantly reduced the metastatic ability of colorectal cancer cells,while inhibiting the expression of KIFC1 so as to stimulate the expression of E-cadherin protein and inhibit the expression of N-cadherin and Vimentin protein(P<0.05).Conclusions Inhibition of the expression of KIFC1 can inhibit the proliferation and metastasis of colorectal cancer cells.