自身抗体的联合检测在自身免疫性肝病诊断中的意义

The significance of combined detection of autoantibodies in the diagnosis of autoimmune liver diseases

目的了解自身抗体的联合检测在自身免疫性肝病诊断中的意义。方法选取2016年1月至2017年12月九江市第一人民医院自身免疫性肝病患者108例(自身免疫性肝病组),病毒性肝炎患者100例(病毒性肝炎组)及50名健康体检者(健康对照组),采用间接免疫荧光法和免疫印迹法检测自身免疫性肝病相关自身抗体。观察自身抗体检出率和不同组别的自身抗体检测结果。结果自身免疫性肝病组自身抗体检出率显著高于病毒性肝炎组和健康对照组(P<0.05),其中ANA、ASMA、AMA-M2和SLA/LP检出率分别为90.7%、36.1%、69.4%和16.7%,均显著高于病毒性肝炎组和健康体检对照组(P<0.05)。结论多项自身抗体联合检测有助于自身免疫性肝病的诊断。

Objective To investigate the significance of combined detection of autoantibodies in the diagnosis of autoimmune liver disease.Methods Choose between January 2016 and December 2017 in Jiujiang City First People's Hospital,108 patients with autoimmune liver disease,autoimmune liver disease group,100 patients with viral hepatitis(viral hepatitis group)and 50 cases of healthy physical examination(healthy control group),using indirect immunofluorescence method and western blot method to detect autoimmune liver disease related autoantibodies.The detection rate of autoantibodies and the detection results of different groups of autoantibodies were observed.Results The detection rate of autoantibodies in autoimmune liver disease group was significantly higher than that in viral hepatitis group and healthy control group(P<0.05).The detection rates of ANA,ASMA,AMA-M2 and SLA/LP were 90.7%,36.1%,69.4%and 16.7%,were significantly higher than those in other two groups(P<0.05).Conclusion Combined detection of autoantibodies related to autoimmune liver diseases contribute to the diagnosis of autoimmune liver diseases.