摘要
试验鉴定获得了猪MMP20基因第3内含子中的2个突变位点g.14334bpC>G和g.14369bpC>T,采用PCR产物直接测序法在大白猪群体中进行基因分型,并对母猪性状进行关联分析,结果发现这两个突变完全连锁,所组成的单倍型显著影响产仔数、产活仔数、21日龄活仔数、出生窝重和出生个体重性状(P<0.05)。GG/CC基因型个体产仔数最低,其次是GC/CT基因型个体,而CC/TT基因型个体产仔数最高。
In the present study two mutations(g.14334bpC>G and g.14369bpC>T)at intron 3 in porcine MMP20 gene were identified by Sanger sequencing.These two mutations were completely linked,and the association results showed that they significantly affected the reproduction traits(TNB,TNA,NAd21,BW and LW)in Large White pigs(P<0.05).GG/CC genotype sows had the lowest litter size,followed by GC/CT genotype sows,while CC/TT genotype sows had the highest litter size.
作者
吴俊静
董斌科
乔木
梅书棋
彭先文
WU Jun-jing;DONG Bin-ke;QIAO Mu;MEI Shu-qi;PENG Xian-wen(Hubei Key Laboratory of Animal Embryo and Molecular Breeding/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《湖北农业科学》
2020年第21期110-112,共3页
Hubei Agricultural Sciences
基金
湖北省重大科技创新项目(2019ABA084、2017ABA134)
湖北省创新群体项目(2018CFA014)
国家生猪产业技术体系项目(CARS-36)
湖北省农业科技创新中心创新团队项目(2019-620-000-001-18)
湖北省创新体系项目(2018skjcx05)。
关键词
猪
繁殖性状
MMP20基因
突变
关联分析
pig
reproduction traits
MMP20 gene
mutation
association analysis
