GC-MS/MS分析中药六方藤和山苦荬中46种禁限用农药残留量

Determination of 46 banned pesticide residues in Cissus hexangularis and Ixeridium chinense by GC-MS/MS

依据风险控制的基本原则,选择中国禁限用、高毒、高风险及中药六方藤和山苦荬种植中常用的农药为检测指标,对不同区域采集的20批中药山苦荬和六方藤样品进行测定。对样品前处理方法、色谱分离、质谱检测条件进行了优化以提高结果的准确性。结果表明,各成分在50~500 ng/mL线性关系良好,相关系数均大于0.99,加标回收率在48.6%~119.0%,符合痕量测定的要求。该方法有针对性地检测了六方藤和山苦荬中高风险、高毒性的农药品种,涵盖了宜用GC-MS/MS检测的绝大多数禁限用农药,检测方法快速、准确,适用于六方藤和山苦荬农药多残留风险控制和日常检测。

The forbidden,restricted,high-toxic,high-risk and customary pesticides were picked out asdetecting indexes according to the principals of risk management.20 batches of Cissus hexangularis and Ixeridium chinense samples collected in different areas were detected.The factors affecting the extraction,purification and detection were optimized and the final condition was established as im⁃prove the accuracy of results.The results showed that the linear relationship of each component in the corresponding range was good in 50~500 ng/mL,the correlation coefficient≥0.99,and the average recovery rate of pesticides was 48.6%~119.0%,which was in line with the trace detection requirements.The detecting indexes in the method cover most of the forbidden and restricted pesticides which are suitable for gas chromatography-tandem mass spectrometry,which is meaningful for the safety supervision of the Cissus hexangula⁃ris and Ixeridium chinense.With the advantage of rapidness and accuracy,this method can be used for routine determination of multipesticides in Cissus hexangularis and Ixeridium chinense.