草果愈伤组织诱导及离体快繁初步研究

Preliminary Study on Callus Induction and Rapid Propagation in Vitro of Amomum tsao-ko Crevost et Lemaire

以草果(Amomum tsao-ko Crevost et Lemaire)茎段和根基部为外植体,MS为基本培养基,探讨不同激素配比的培养基对草果不定芽的诱导、愈伤诱导增殖分化的影响。结果表明:①灭菌时长对草果的不定芽增殖分化有一定影响,茎段灭菌8min,根基部12min,增殖分化效果较好。选择外植体时,接种保留一层叶鞘的茎段要比接种裸露茎段增殖效果要好。②MS+6-BA 6mg/L+2,4-D 2mg/L的培养基对不定芽增殖分化效果最好,不定芽叶色鲜绿,苗健壮,整体感最佳。

In this study,the stems and rhizomes of Amomum tsao-ko Crevost et Lemaire were used as explants on callus induction and rapid propagation in vitro,and MS as the basal medium,so as to investigate the effects of different hormone combinations on induction of adventitious buds,callus-induced proliferation and differentiation of Amomum tsao-ko Crevost et Lemaire.The results showed that the duration of sterilization had a certain degree of influence on the proliferation and differentiation of adventitious buds.The stems were sterilized for 8 minutes,while the rhizomes were sterilized for 12 minutes.Thus,the proliferation and differentiation effect was relatively better.When the explants were selected,it was better to inoculate a stem with a layer of leaf sheath than inoculate a bare stem.The medium of MS+6-BA 6mg/L+2,4-D 2mg/L had the optimal effect on proliferation and differentiation of adventitious bud.The adventitious bud leaves were bright green,the seedlings were robust,and the overall perception was well.