摘要
采用超声乳化聚合法制备了聚甲基丙烯酸丁酯纳凝胶,然后经结晶致孔法制备了内嵌纳凝胶的甲基丙烯酸羟乙酯纳晶胶,对其接枝功能单体N,N,N-三甲基乙烯基苯甲氯化铵,得到内嵌纳凝胶阴离子交换纳晶胶,用于从发酵液中分离苯乳酸。结果表明:所得纳晶胶具有良好的渗透性能和轴向分散性能,在流速为1 cm·min-1时,不同配比纳晶胶对牛血清白蛋白的吸附容量为3.9~5.4 mg·ml-1;将质量比为8∶2(HEMA∶pBMA)的纳晶胶用于从转化液中分离苯乳酸,所得苯乳酸的纯度达89.24%,回收率93.74%,分离效果好,表明其在生物分离方面有良好的应用前景。
Hydrophobic nanogels were synthesized by emulsion polymerization method under ultrasound using butyl methacrylate as the monomer.Then,anion exchange nano-cryogels embedded with the obtained nanogels were prepared by cryo-polymerization using poly(2-hydroxyethyl methacrylate)as the monomer,followed by a graft polymerization with the monomer(vinylbenzyl)trimethyl ammonium chloride.The anion exchange nano-cryogels were used to isolate phenyllactic acid from biotransformation broth.The results show that the obtained nanocrystalline gel has good permeability and axial dispersion performance.When the flow rate is 1 cm·min^-1,the adsorption capacity of different ratios of nanocrystalline gel for bovine serum protein is 3.9—5.4 mg·ml^-1.When the nano-cryogel with mass ratio of 8∶2(HEMA∶pBMA)was used to isolate phenyllactic acid from transformation broth,phenyllactic acid with high purity of 89.24%and recovery of 93.74%was obtained,indicating that the nanocryogels could have potential applications in bioseparation.
作者
贺亚维
张颂红
黄杰
刘流
李国华
贠军贤
HE Yawei;ZHANG Songhong;HUANG Jie;LIU Liu;LI Guohua;YUN Junxian(Shaanxi Energy Institute,Xianyang 712000,Shaanxi,China;State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology,College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310032,Zhejiang,China)
出处
《化工学报》
EI
CAS
CSCD
北大核心
2020年第12期5636-5643,共8页
CIESC Journal
基金
国家自然科学基金项目(21576240)
浙江省自然科学基金项目(LY16B060011,LZ14B060001)。
关键词
纳凝胶
纳晶胶
吸附
苯乳酸
nanogels
nano-cryogels
adsorption
phenyllactic acid