摘要
Fusarium crown rot(FCR),a chronic and severe disease caused by various Fusarium species,is prevalent in semi-arid cropping regions worldwide.One of the major QTL conferring FCR resistance was detected on chromosome arm 1 HL(Qcrs.cpi-1 H)in barley.To develop markers that can be reliably used to incorporate the resistance locus into breeding programs,we developed and assessed a near-isogenic line-derived population consisting of1180 recombinant inbred lines targeting the locus.Using this population,we delineated Qcrs.cpi-1 H into an interval of 0.4 c M covering a physical length of about 487 kb.Six markers co-segregating with this locus were generated.Co-linearity for genes located in this interval between the genome of barley and those of either rice or Brachypodium distachyon is poor.Three genes with non-synonymous variations between the resistant and susceptible lines were identified within the interval.The results reported in this study not only provide markers for integrating Qcrs.cpi-1 H into breeding programs,but also form a solid foundation for cloning the causal gene(s)underlying this locus.
基金
partially supported by the Grains Research and Development Corporation,Australia(CFF00010)
University of Tasmania,Australia
the China Scholarship Council for financial supports。
