高压氧暴露协同骨髓间充质干细胞移植对卵巢早衰大鼠卵巢功能重建的研究

Ovarian functional reconstruction in rats with premature ovarian failure through hyperbaric oxygen combined with bone marrow mesenchymal stem cell transplantation

目的:探讨高压氧(hyperbaric oxygen,HBO)协同骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植对卵巢早衰(premature ovarian failure,POF)大鼠卵巢功能的重建,探究其治疗机制。方法:雌性大鼠采用腹腔注射环磷酰胺(cyclopho sphamide,CTX)建立卵巢早衰模型。造模成功后随机分为3组(模型组、BMSCs移植组、HBO+BMSCs移植组),每组各12只;另取空白组12只。BMSCs移植组、HBO+BMSCs移植组给予不同干预后,分别记录大鼠动情周期,测定血清E2、FSH激素水平,各级卵泡计数,观察卵巢显微结构、移植细胞在体内存活分布、生殖能力情况,评价卵巢功能。结果:HBO+BMSCs移植组动情周期恢复进程优于移植组;HBO+BMSCs移植组E2水平(82.463±4.108)较BMSCs移植组(77.233±3.714)升高(P=0.047);HBO+BMSCs移植组FSH水平(21.292±2.458)较BMSCs移植组(24.574±2.389)降低(P=0.043);HBO+BMSCs移植组GF(11.50±1.27)、MF(4.50±0.53)数量较BMSCs移植组(13.50±1.43、5.40±0.70)呈不同程度增加(P=0.024,P=0.028),HBO+BMSCs移植组PF(46.00±3.74)、AF(16.10±2.18)较BMSCs移植组(41.30±3.20、13.30±1.89)呈不同程度减少(P=0.044,P=0.040)。HBO+BMSCs移植组在BMSCs移植30 d后可见到绿色荧光表达多于BMSCs移植组;HBO+BMSCs移植组大鼠娩出乳鼠多于BMSCs移植组。结论:HBO联合BMSCs移植具有协同的、安全有效的促进卵巢功能重建的作用。

Objective:To study the ovarian functional reconstruction in rats with premature ovarian failure(POF)through hyperbaric oxygen(HBO)combined with bone marrow mesenchymal stem cells(BMSCs)transplantation.Methods:Female sprague dawley(SD)rats were subjected to intraperitoneal injection of cyclopho sphamide(CTX)to induce POF.Successful modeling(36 rats)were randomly divided into the model group,the BMSCs transplantation group,and HBO+BMSCs transplantation group,12 in each group.Another 12 rats were taken from the blank group.After the BMSCs transplantation group and the HBO+BMSCs transplantation group were given individual intervention,rats estrus cycle,serum E2,FSH hormone level,the amount of follicles at all levels were recorded respectively,and ovarian microstructure,distribution of transplanted cells in ovarian tissue and reproductive ability were observed so as to evaluate the ovarian function.Results:The recovery of estrus cycle in the HBO+BMSCs transplantation group was better than that in the BMSCs transplantation group.E2 in the HBO+BMSCs transplantation group(82.463±4.108)was higher than that in the BMSCs transplantation group(77.233±3.714)(P=0.047)and FSH in the HBO+BMSCs transplantation group(21.292±2.458)was lower than that in the BMSCs transplantation group(24.574±2.389)(P=0.043).Compared with the BMSCs transplantation group(13.50±1.43),(5.40±0.70),the number of GF(11.50±1.27)MF(4.50±0.53)in the HBO+BMSCs transplantation group increased at varying degrees(P=0.024,0.028).Compared with the BMSCs transplantation group(41.30±3.20,13.30±1.89),the number of PF(46.00±3.74),AF(16.10±2.18)in the HBO+BMSCs transplantation group decreased at varying degrees(P=0.044,0.040).The expression of green fluorescence in the HBO+BMSCs transplantation group was more than that in transplantation group after 30 days of transplantation.SD rats in the HBO+BMSCs transplantation group had more offspring than that in the BMSCs transplantation group.Conclusion:The HBO+BMSCs transplantation has a synergistic effect on functional reconstruction of POF,and it may play a safe and effective role in the treatment of POF.