氟西汀对慢性不可预见应激模型大鼠前额叶皮质磷脂酰乙醇胺的影响

Impact of fluoxetine on the molecules of phosphatidylethanolamine in prefrontal cortex of rats with chronic unpredictable stress

目的探讨氟西汀对慢性不可预见应激(CUS)模型大鼠前额叶皮质磷脂酰乙醇胺(PE)的影响。方法按随机数字表法将18只SD大鼠随机分为对照组(Sham组)、模型组(CUS组)和氟西汀组(Flx组)。CUS组和Flx组均接受CUS造模,并且在造模后接受生理盐水(1 ml/kg)或氟西汀(10 mg/kg)腹腔注射,连续14 d;Sham组不进行CUS造模,但是每天接受腹腔注射生理盐水。14 d后处死大鼠,取前额叶皮质进行脂质组学分析,比较各处理组前额叶皮质总的PE和PE小分子相对丰度差异。结果(1)与Sham组[(10.50±7.32)×10^11]比较,CUS组PE相对丰度[(11.95±8.46)×10^11]明显减低(P<0.01),而Flx组[(10.31±9.16)×10^11]差异则无统计学意义(P>0.05);(2)与Sham组比较,CUS组3个PE小分子相对丰度减低,分别为PE(36:4p)(68.67±14.68)、PE(38:3p)(49.73±19.55)、PE(38:4p)(76.10±7.84),差异均有统计学意义(均P<0.05);(3)与CUS组比较,Flx组6个PE小分子相对丰度减低,包括PE(36:4)(72.53±10.22)、PE(36:2)(87.86±8.26)、PE(38:5p)(75.66±9.53)、PE(38:5)(89.93±9.79)、PE(38:4)(86.55±5.40)、PE(40:7p)(81.57±7.55),差异均有统计学意义(均P<0.05);(4)与Sham组比较,Flx组6个PE小分子相对丰度减低,包括PE(38:4p)(75.85±5.63)、PE(38:3p)(44.22±12.61)、PE(38:4)(82.49±9.41)、PE(40:5p)(78.01±5.31)、PE(36:4)(76.00±6.34)、PE(38:6)(77.45±13.06),差异均有统计学意义(均P<0.05)。结论CUS模型大鼠前额叶皮质内PE水平异常,氟西汀可以调节CUS模型大鼠前额叶皮质的PE水平。

Objective To investigate the impact of fluoxetine on the compositions of phosphatidylethanolamine(PE)in prefrontal cortex(PFC)of rats with chronic unpredictable stress(CUS).Methods A total of 18 SD rats were randomly divided into control group(Sham),model group(CUS)and fluoxetine group(Flx)by random number table method.CUS group and Flx group received CUS stimulus,and each group received intraperitoneal injection of normal saline(1 ml/kg)or fluoxetine(10 mg/kg)for 14 consecutive days,while Sham group did not received CUS,but received intraperitoneal injection of normal saline every day.The rats were killed after 14 days and the PFC was isolated for lipomics analysis.The relative concentrations of total PE and different molecules in the PFC of each group were compared.Results(1)Compared to Sham group[(10.50±7.32)×10^11],the relative concentrations of PE in the CUS group[(11.95±8.46)×10^11]was significantly reduced(P<0.01),while the difference to Flx group[(10.31±9.16)×10^11]was not significant(P>0.05).(2)Compared to Sham group,the relative concentrations of 3 PE small molecules decreased in CUS group,which are PE(36:4p)(68.67±14.68),PE(38:3p)(49.73±19.55),PE(38:4p)(76.10±7.84)respectively,with statistical significance(P<0.05).(3)Compared to CUS group,the relative concentrations of 6 PE small molecules decreased in Flx group,which are PE(36:4)(72.53±10.22),PE(36:2)(87.86±8.26),PE(38:5p)(75.66±9.53),PE(38:5)(89.93±9.79),PE(38:4)(86.55±15.40),PE(40:7p)(81.57±7.55)respectively,with statistical significance(P<0.05).(4)Compared to Sham group,the relative concentrations of 6 PE small molecules decreased in Flx group,which are PE(38:4p)(75.85±5.63),PE(38:3p)(44.22±12.61),PE(38:4)(82.49±9.41),PE(40:5p)(78.01±5.31),PE(36:4)(76.00±6.34),PE(38:6)(77.45±13.06),with statistical significance(P<0.05).Conclusions Fluoxetine can regulate the level of PE in prefrontal cortex of CUS model rats.