静电纺明胶/壳聚糖可食用纳米纤维膜的制备、表征及其抑菌特性研究

Preparation,Characterization and Antibacterial Properties ofElectrospun Gelatin/Chitosan Edible Nanofiber Membrane

为开发新型可食用食品抗菌包装材料,本研究以明胶、壳聚糖天然物质为原料,通过静电纺丝技术,分别制备明胶/壳聚糖=6∶1、8∶1、10∶1的可食纳米抗菌膜。并对抗菌膜进行表征及抑菌特性进行分析。对静电纺丝共混液的黏度进行流变分析,得出明胶与壳聚糖的比例为6∶1时共混液的黏度最高,傅里叶红外光谱和X射线衍射的结果显示,不同配比的明胶壳聚糖抗菌膜的结构未发生化学改变,差示扫描量热和热重分析的结果显示,明胶与壳聚糖的比例为6∶1时的热力学性能显著优于其他两种配比的抗菌膜。对三个配比的抗菌膜进行平板抑菌、扫描电镜观察试验,结果显示三种配比的抗菌膜均有抑菌活性,以明胶与壳聚糖的比例为6∶1处理组的抑菌效果最明显。经过以上实验得出,当明胶:壳聚糖的比例为6∶1时,抗菌膜的稳定性和抗菌活性最优,可以作为优良的抗菌包装材料。

In order to develop a new type of edible food antibacterial packaging material,this research used gelatin and chitosan as raw materials,and edible nano antibacterial membranes was prepared with gelatin/chitosan=6∶1,8∶1,10∶1 respectively through electrostatic spinning technology.Rheological analysis of the viscosity of the electrospinning blend solution showed that it was the highest when the ratio of gelatin to chitosan was 6∶1.Besides,the preparation,characterization and antibacterial properties of the antibacterial membrane were studied.The results of Fourier transform infrared spectroscopy and X-ray diffraction showed that the structure of the gelatin chitosan antibacterial membranes with different proportions didn’t change chemically.The results of differential scanning calorimetry and thermogravimetric analysis showed that the ratio of gelatin to chitosan was at 6∶1 when the thermodynamic performance was significantly better than the other two types of antibacterial membranes.Three antibacterial films with plate antibacterial,scanning electron microscopy,and laser confocal antibacterial effect observation tests were performed.The results showed that the three antibacterial films had antibacterial activity.The ratio of gelatin to chitosan was 6∶1 that treatment group had the most significant antibacterial effect.The above experiments showed that when the ratio of gelatin:chitosan was 6∶1,the stability and antibacterial activity of the antibacterial film was optimal,and it could be used as an excellent antibacterial packaging material.