基于网络药理学探究补阳还五汤防治动脉粥样硬化的作用机制

Network pharmacology-based identification of key mechanism of Buyang Huanwu decoction acting on atherosclerosis

目的挖掘补阳还五汤防治动脉粥样硬化的物质基础与作用机制。方法以中医药大数据的信息技术为手段,基于中药系统药理学平台,以人类表型本体数据库为支撑,对补阳还五汤防治动脉粥样硬化进行关联分析。构建“补阳还五汤-中药-化学成分-核心靶点-关键通路”的可视化网络,探讨补阳还五汤防治动脉粥样硬化的物质基础和作用机制。结果利用中药系统药理学平台筛选出补阳还五汤的140个化合物中的174个核心靶点。核心靶点MAPK1、PIK3R1、SRC、AKT1、APP、HRAS、JUN、TP53、EGF、RELA等与动脉粥样硬化的关键靶点相互关联,参与调控了胆固醇外排、细胞凋亡等生物过程,并涉及癌症、乙型肝炎,PI3K-Akt通路、T细胞受体信号传导通路,以及FoxO通路等在内的炎症和免疫相关通路。结论补阳还五汤可能通过参与调控脂质代谢、细胞凋亡、以及调节免疫和炎症反应等机制防治动脉粥样硬化。

Aim To explore the effective components and inhibitory mechanism of Buyang Huanwu decoction in atherosclerotic progress.Methods TCM system pharmacology platform and Human Phenotypic Ontology database by means of information technology of TCM big data were carried out to screen the association between Buyang Huanwu decoction and atherosclerosis.A visual network of“Buyang Huanwu Decoction-Traditional Chinese Medicine-Chemical Components-Core Target-Critical Pathway”was constructed.The inhibitory effect of Buyang Huanwu decoction on atherosclerosis was explored by the effective components and mechanism analysis.Results The 174 core targets from 140 compounds of Buyang Huanwu decoction were screened by pharmacology platform of traditional Chinese medicine system.They are involved in biological processes such as cholesterol efflux,apoptosis,inflammation and immune-related pathway such as cancer,Hepatitis B,PI3K-Akt pathway,T cell receptor signal pathway and FoxO pathway.Conclusions Buyang Huanwu decoction may be involved in the regulation of lipid metabolism,apoptosis,immune and inflammatory responses and other mechanisms to prevent and treat atherosclerosis.