黑豆中总皂苷提取工艺的研究

Study on Extraction Technology of Total Saponins from Black Bean

研究黑豆总皂苷的最佳提取工艺;采用紫外分光光度法测定总皂苷提取率,根据单因素试验考察提取体系的乙醇浓度、提取时间、液固比、提取次数以及水浴温度对总皂苷提取率的影响,采用响应面法优化最佳提取条件。优化得到的最佳提取工艺为:乙醇浓度70%,液固比9∶1(mL/g),提取2次,提取时间1 h、水浴温度85℃,总皂苷提取率为3.33%;响应面法优化黑豆总皂苷提取工艺预测性好,可信度高。

The optimal extraction technology of total saponins from black bean was studied in the paper.The total saponins was determined by ultraviolet spectrophotometry.The effects of ethanol concentration,extraction time,liquid-to-material ratio,extraction times and water bath temperature on the extraction rate of total saponins were investigated through single-factor experiments.The optimal extraction condition was optimized by response surface methodology.Ultimately,the results were as follows:the ethanol concentration 70%,liquid-to-material ratio 9∶1(mL/g),extraction 2 times,extraction time 1 h,water bath temperature 85℃.In this condition,the extraction rate of total saponins was 3.33%.The extraction technology of total saponins from black bean which was optimized through response surface methodology was in high credibility and good predictability.