不同免疫学检验方法对于结核病的临床价值研究

Study on the Clinical Value of Different Immunological Testing Methods for Tuberculosis

目的分析不同免疫学检验方法,探索有效的结核病临床诊断方案。方法选取2019年10月至2020年4月因结核病而入潍坊市脑科医院治疗的90例患者为研究对象纳入结核组,同期另选取90例非结核患者作为对照组。同时采用蛋白芯片法与胶体金法进行检测并比较两种检测方法的差异性,分析不同检测方法在结核病辅助诊断当中的临床价值。结果对于结核组患者来说,其胶体金法阳性反应率数据达到了71.11%,而对照组的胶体金法阳性反应率数据为7.80%,结核组显著优于对照组,且差异有统计学意义(P<0.05);另外,阿拉伯甘露糖脂(LAM)、结核杆菌16 kD(蛋白16 kD)和结核杆菌38 kD(蛋白38 kD)数据中,结核组蛋白芯片法阳性反应率分别是47.78%(43/90)、26.67%(24/90)、46.67%(42/90),而对照组蛋白芯片法阳性反应率分别是8.90%(8/90)、0.00%(0/90)、8.90%(8/90),结核组显著优于对照组,且胶体金法免疫检验优于蛋白芯片法免疫检验,差异有统计学意义(P<0.05)。结论胶体金法对于结核疾病的诊断有着显著的优势,该方式可在以后工作中实施广泛的推广。

Objective To analyze different immunological testing methods and explore effective clinical diagnosis programs for tuberculosis.Methods Select 90 patients who were admitted to Weifang Brain Hospital for tuberculosis between October 2019 and April 2020 as materials,and implement differentiated immunological examination methods,namely protein chip method and colloidal gold method,to record the differences.The positive reaction rate data of tuberculosis under the test methods provide a theoretical basis for the follow-up clinical diagnosis of tuberculosis.Results For patients in the tuberculosis group,the positive reaction rate of the colloidal gold method reached 71.11%,while the positive reaction rate of the colloidal gold method of the control group was 7.80%.The tuberculosis group was significantly better than the control group,and the difference was statistically significant(P<0.05);In addition,in the data of LAM,protein 16 kD and protein 38 kD,the positive reaction rate of tuberculosis histone chip method was 47.78%(43/90),26.67%(24/90),and 46.67%(42/90),while the positive reaction rate of the protein chip method in the control group was 8.90%(8/90),0.00%(0/90),8.90%(8/90).The tuberculosis group was significantly better than the control Group,and the colloidal gold immunoassay due to the protein chip immunoassay,there is a statistically significant difference in the data(P<0.05).Conclusion The colloidal gold method has significant advantages for the diagnosis of tuberculosis,and this method can be widely promoted in future work.